[一种测定固醇代谢改变的酿酒酵母中维生素原D水平的方法]
[A method of determining the level of provitamin D in Saccharomyces cerevisiae with altered sterol metabolism].
作者信息
Chichikalova M M, Zhakovskaia Z A, Moseĭchuk A V, Mikhalova N P, V'iunov K A
出版信息
Prikl Biokhim Mikrobiol. 1987 Sep-Oct;23(5):703-5.
PMID:3321030
Abstract
A method is proposed that allows to choose among the polyene-resistant mutants of the yeasts Saccharomyces cerevisiae with changed biosynthesis of ergosterol promising producers of provitamin D4 and structural analogs of provitamin D3. The method involves both UV-spectrophotometry and thin-layer chromatography. The results obtained are supported by the data of mass-spectrometry of sterols.
摘要
提出了一种方法，该方法能够从酿酒酵母的多烯抗性突变体中进行选择，这些突变体的麦角固醇生物合成发生了变化，有望成为维生素D4原以及维生素D3原结构类似物的生产者。该方法涉及紫外分光光度法和薄层色谱法。所获得的结果得到了固醇质谱数据的支持。
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