Chao Jianbin, Duan Yuexiang, Liu Yaoming, Xu Miao, Zhang Yongbin, Huo Fangjun, Zhang Ting, Wang Juanjuan, Yin Caixia
Scientific Instrument Center, Shanxi University, Taiyuan 030006, PR China.
Scientific Instrument Center, Shanxi University, Taiyuan 030006, PR China; School of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, PR China.
Spectrochim Acta A Mol Biomol Spectrosc. 2020 Dec 15;243:118813. doi: 10.1016/j.saa.2020.118813. Epub 2020 Aug 11.
Detection of hypochlorite ions (ClO) in the organisms is of great significance for finding effective treatments for inflammations and diseases. Recently, fluorescent probes have aroused wide public concern as one of the effective tools for detecting molecules and ions. Nevertheless, due to low sensitivity and poor biocompatibility, the effect of fluorescent probes for biological imaging is still not ideal. For this, we developed a novel ratiometric fluorescent probe, 7-(diethylamino)-3-((E)-3-(9-ethyl-9H-carbazol-3-yl)acryloyl)-2H-chromen-2-one (DCC), which could be used for colorimetric detection of ClO. Study showed that, the detection mechanism of DCC is that probe can be rapidly oxidized to an enoic acid by ClO, resulting in a series of changes in spectral properties. This mechanism was confirmed experimentally and verified by theoretical calculations. It is worth mentioning that DCC has not only been successfully applied to the detection of exogenous and endogenous OCl in living cells, but also used for the detection of ClO in zebrafish, and Arabidopsis.
检测生物体内的次氯酸根离子（ClO）对于寻找炎症和疾病的有效治疗方法具有重要意义。近年来，荧光探针作为检测分子和离子的有效工具之一引起了广泛关注。然而，由于灵敏度低和生物相容性差，荧光探针用于生物成像的效果仍不理想。为此，我们开发了一种新型比率荧光探针7-(二乙氨基)-3-((E)-3-(9-乙基-9H-咔唑-3-基)丙烯酰基)-2H-色烯-2-酮（DCC），可用于比色法检测ClO。研究表明，DCC的检测机制是探针可被ClO迅速氧化为烯酸，导致光谱性质发生一系列变化。这一机制通过实验得到证实，并经理论计算验证。值得一提的是，DCC不仅已成功应用于活细胞中外源和内源性OCl的检测，还用于斑马鱼和拟南芥中ClO的检测。
