School of Ophthalmology & Optometry, School of Biomedical Engineering, Wenzhou Medical University, Wenzhou, Zhejiang, 325035, China.
Engineering Research Center of Clinical Functional Materials and Diagnosis & Treatment Devices of Zhejiang Province, Wenzhou Institute, University of Chinese Academy of Sciences, Wenzhou, Zhejiang, 325001, China.
Anal Chim Acta. 2021 May 1;1157:338391. doi: 10.1016/j.aca.2021.338391. Epub 2021 Mar 12.
Tracking and quantifying hypochlorite (ClO) in biological systems and environments remain challenging tasks, and many efforts have been made to improve ClO recognition performance by modifying the sensor structure. In this study, a pre-designed coumarin/furanohydrazide-based sensor (CMFH) with the coumarin moiety as the building block (fluorogen) was rationally prepared as a ratiometric and colorimetric chemosensor for ClO recognition. As expected, CMFH demonstrated excellent sensitivity and selectivity for ClO detection. The fluorescence signal ratio (F/F) showed strong ClO dependency, and the sensor exhibited ultrafast detection (within 60 s) and a low detection limit of 563 nM. Due to its low cytotoxicity and good tissue permeability, CMFH was demonstrated as a dual-channel sensor for ClO bioimaging and visualization in cells, zebrafish, and even bacteria. Furthermore, CMFH-loaded paper strips were successfully applied to the colorimetric and fluorescent visualization of ClO. The results demonstrate that CMFH has potential application value for tracking ClO in various biosystems and environments.
在生物体系和环境中追踪和定量检测次氯酸根（ClO）仍然是具有挑战性的任务，许多研究致力于通过修饰传感器结构来提高 ClO 识别性能。在本研究中，设计了一种基于香豆素/呋喃甲酰肼的传感器（CMFH），以香豆素部分作为构建块（荧光团），作为比率型和比色型 ClO 识别化学传感器。预期 CMFH 对 ClO 的检测表现出优异的灵敏度和选择性。荧光信号比（F/F）显示出对 ClO 的强依赖性，传感器具有超快的检测速度（在 60 秒内）和低检测限 563 nM。由于其低细胞毒性和良好的组织通透性，CMFH 被证明是一种双通道传感器，可用于细胞、斑马鱼甚至细菌中的 ClO 生物成像和可视化。此外，CMFH 负载的纸条成功地应用于 ClO 的比色和荧光可视化。结果表明，CMFH 具有在各种生物体系和环境中追踪 ClO 的潜在应用价值。
