Dogan Belgin, Zhang Shiying, Kalla Sarah E, Dogan Esra I, Guo Cindy, Ang Chelston R, Simpson Kenneth W
Department of Clinical Sciences, College of Veterinary Medicine, Cornell University, Ithaca, NY 14853, USA.
Antibiotics (Basel). 2020 Aug 25;9(9):540. doi: 10.3390/antibiotics9090540.
Invasive is causally associated with granulomatous colitis (GC) of Boxer dogs and French Bulldogs. The virulence determinants of GC are unclear. isolated from 16 GC (36 strains) and 17 healthy control (HC: 33 strains) dogs were diverse in phylogeny, genotype, and serotype and lacked diarrheagenic genes. Genes encoding type II (), IV (), and VI () secretion systems, long polar fimbriae (154/141), and iron acquisition (, ) were frequent in GC and HC. from 14/15 GC and 10/11 HC invaded Caco-2 better than non-pathogenic strain DH5α, with invasion correlated with motility and presence of and . from all GC and 10/11 HC survived better than DH5α in J774 macrophages, with adherent-invasive (AIEC) in 60% GC and 73% HC. AIEC replicated in monocyte derived macrophages from a GC Boxer with CD48/SLAM risk haplotype but not the HC. Fluroquinolone resistant were less motile and invasive than fluoroquinolone sensitive ( < 0.05), and only 1/8 resistant strains met criteria for AIEC. In conclusion GC are diverse, resemble extraintestinal pathogenic (ExPEC), including AIEC, and can replicate in GC-susceptible macrophages. They are likely resident pathosymbionts that can opportunistically persist within macrophages of a GC-susceptible dog.
侵袭性与拳师犬和法国斗牛犬的肉芽肿性结肠炎（GC）存在因果关联。GC的毒力决定因素尚不清楚。从16只患有GC的犬（36株菌株）和17只健康对照犬（HC：33株菌株）中分离出的菌株在系统发育、基因型和血清型方面具有多样性，并且缺乏致腹泻基因。编码II型（）、IV型（）和VI型（）分泌系统、长极毛（154/141）以及铁摄取（，）的基因在GC和HC中很常见。来自14/15只患有GC的犬和10/11只健康对照犬的菌株比非致病性菌株DH5α对Caco-2细胞的侵袭性更强，侵袭与运动性以及和的存在相关。来自所有患有GC的犬和10/11只健康对照犬的菌株在J774巨噬细胞中的存活能力比DH5α更强，60%的GC犬和73%的健康对照犬中存在黏附侵袭性大肠杆菌（AIEC）。AIEC在一只具有CD48/SLAM风险单倍型的GC拳师犬的单核细胞衍生巨噬细胞中复制，但在健康对照犬中不复制。耐氟喹诺酮的菌株比氟喹诺酮敏感的菌株运动性和侵袭性更低（<0.05），并且只有1/8的耐药菌株符合AIEC的标准。总之，GC菌株具有多样性，类似于包括AIEC在内的肠道外致病性大肠杆菌（ExPEC），并且可以在对GC易感的巨噬细胞中复制。它们可能是常驻的病理共生菌，能够在对GC易感的犬的巨噬细胞中机会性地持续存在。
