Versura P, Maltarello M C, Caramazza R, Laschi R
Department of Ophthalmology, University of Bologna, Italy.
Curr Eye Res. 1988 Mar;7(3):277-86. doi: 10.3109/02713688809047033.
We studied 8 soft contact lenses from asymptomatic wearers by means of an immunocytochemical method, in transmission electron microscopy. In particular, the presence of IgA, IgG, IgE, Clq complement fraction within the surface deposits was analyzed. All the lenses were found positive for the immunoglobulins and the Clq, being the tarsal side more heavily coated than the corneal one. IgA was the predominant Ig, followed by IgG, IgE, and Clq in this descending order. New, never worn lenses were found completely negative for any of the proteins under investigation. We conclude that the Igs come from the tear fluid and speculate about the Clq as a possible sign of involvement of the host immuno-defense mechanism against the prosthesis.
我们通过免疫细胞化学方法，在透射电子显微镜下研究了8名无症状佩戴者的软性隐形眼镜。特别分析了表面沉积物中IgA、IgG、IgE、Clq补体成分的存在情况。所有镜片的免疫球蛋白和Clq均呈阳性，睑板侧的涂层比角膜侧更厚。IgA是主要的免疫球蛋白，其次是IgG、IgE和Clq，呈递减顺序。新的、从未佩戴过的镜片对所研究的任何蛋白质均呈完全阴性。我们得出结论，免疫球蛋白来自泪液，并推测Clq可能是宿主针对假体的免疫防御机制参与的一个迹象。
