Department of Hematology, The First Affiliated Hospital of Nanjing Medical University, Jiangsu Province Hospital, 300 Guangzhou Road, Nanjing, 210029, Jiangsu Province, China.
Department of Cancer Institute, Zhenjiang Clinical Medical College of Nanjing Medical University, The Affiliated People's Hospital of Jiangsu University, 8 Dianli Road, Zhenjiang 212002, Jiangsu Province, China.
Biosci Rep. 2020 Sep 30;40(9). doi: 10.1042/BSR20201590.
Circular RNAs (circRNAs) may be potential biomarkers or therapeutic targets of hemophagocytic syndrome (HPS) due to their high stability, covalently closed structure and implicated roles in gene regulation. The aim of the present study was to determine and characterize the circRNAs from natural killer (NK)/T-cell lymphoma-associated hemophagocytic syndrome (NK/T-LAHS). CircRNA in NK/T-LAHS and healthy control patient serum were assessed using next-generation sequencing (NGS). One hundred and forty-three differentially expressed circRNAs of which 114 were up-regulated and 29 were down-regulated in NK/T-LAHS patients were identified. Next, Gene Ontology (GO) function and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses to explore the roles of these circRNAs were utilized, and a microRNA (miRNA) target gene prediction software to predict the interaction of circRNAs and miRNAs was used. Moreover, five circRNAs were then selected as NK/T-LAHS candidate circRNAs which were related to tumors and contained NK/T-LAHS-related miRNA-binding sites. Using real-time PCR, the significant up-regulation of these five circRNAs in NK/T-LAHS patient serum were verified. Together these results show that circRNAs may serve as valuable diagnostic biomarkers of early NK/T-LAHS, with potential therapeutic targets in disease progression.
环状 RNA（circRNA）由于其高稳定性、共价闭合结构以及在基因调控中的作用，可能成为噬血细胞综合征（HPS）的潜在生物标志物或治疗靶点。本研究旨在确定并描述自然杀伤（NK）/T 细胞淋巴瘤相关噬血细胞综合征（NK/T-LAHS）中的 circRNA。使用下一代测序（NGS）评估 NK/T-LAHS 和健康对照患者血清中的 circRNA。在 NK/T-LAHS 患者中鉴定出 143 个差异表达的 circRNA，其中 114 个上调，29 个下调。接下来，利用基因本体论（GO）功能和京都基因与基因组百科全书（KEGG）通路分析来探讨这些 circRNA 的作用，并使用 miRNA 靶基因预测软件来预测 circRNA 和 miRNA 的相互作用。此外，还选择了五个与肿瘤相关且含有 NK/T-LAHS 相关 miRNA 结合位点的 circRNA 作为 NK/T-LAHS 的候选 circRNA。通过实时 PCR，验证了这五个 circRNA 在 NK/T-LAHS 患者血清中的显著上调。这些结果表明，circRNA 可能作为早期 NK/T-LAHS 的有价值的诊断生物标志物，具有疾病进展中的潜在治疗靶点。
