肺鳞癌环状 RNA 表达谱：潜在生物标志物和治疗靶点的鉴定。
Circular RNA expression profile of lung squamous cell carcinoma: identification of potential biomarkers and therapeutic targets.
机构信息
Department of Medical Oncology, Qilu Hospital, Shandong University, Jinan, Shandong 250012, China.
Department of Otorhinolaryngology Head and Neck Surgery, Shandong Provincial ENT Hospital, Shandong University, Jinan, Shandong 250021, China.
出版信息
Biosci Rep. 2020 Apr 30;40(4). doi: 10.1042/BSR20194512.
Emerging evidences indicated that exosomal circular RNAs (circRNAs) could serve as diagnostic biomarkers for cancers. However, the expression profiles and clinical significance of circRNAs in lung squamous cell carcinoma (LUSC) remain largely unknown. Herein, we analyzed circRNAs expression profile in six pairs of plasma exosome samples of LUSC patients using high-throughput sequencing. A total of 252 differentially expressed exosomal circRNAs were identified, including 133 up-regulated circRNAs and 119 down-regulated circRNAs. Subsequently, the circRNAs-miRNAs-mRNAs interaction network was built to investigate potential function of circRNAs. Three up-regulated circRNAs (hsa_circ_0014235, hsa_circ_0025580 and hsa_circ_0026403) were implied to participate in cancer-related pathways. QRT-PCR experiment confirmed the up-regulation of hsa_circ_0014235 and hsa_circ_0025580. Finally, clinical studies indicated that hsa_circ_0014235 and hsa_circ_0025580 could serve as novel diagnostic biomarkers for LUSC. Taken together, our study revealed exosomal circRNAs expression profile in LUSC for the first time and showed the important diagnostic potential for circRNAs in LUSC.
新出现的证据表明，外泌体环状 RNA（circRNA）可以作为癌症的诊断生物标志物。然而，肺鳞状细胞癌（LUSC）中 circRNA 的表达谱和临床意义在很大程度上仍然未知。在此，我们使用高通量测序分析了 6 对 LUSC 患者血浆外泌体样本中的 circRNA 表达谱。共鉴定出 252 个差异表达的外泌体 circRNA，包括 133 个上调的 circRNA 和 119 个下调的 circRNA。随后，构建了 circRNA-miRNA-mRNA 相互作用网络，以研究 circRNA 的潜在功能。三个上调的 circRNA（hsa_circ_0014235、hsa_circ_0025580 和 hsa_circ_0026403）被暗示参与癌症相关途径。实时荧光定量 PCR 实验证实了 hsa_circ_0014235 和 hsa_circ_0025580 的上调。最后，临床研究表明 hsa_circ_0014235 和 hsa_circ_0025580 可以作为 LUSC 的新型诊断生物标志物。总之，我们的研究首次揭示了 LUSC 中外泌体 circRNA 的表达谱，并显示了 circRNA 在 LUSC 中重要的诊断潜力。
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