Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Menoufia, 32897, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, University of Sadat City, Sadat City, Menoufia, 32897, Egypt.
Environ Sci Pollut Res Int. 2021 Jan;28(2):1762-1774. doi: 10.1007/s11356-020-10588-4. Epub 2020 Aug 27.
The current study aimed to investigate the protective effect of corn silk methanolic extract (CSME) against acetaminophen (APAP)-induced nephrotoxicity. The present study was carried out on 40 male Wistar albino rats, which were randomly divided into four groups (n = 10): control group, orally administered with a single dose of 1.8 ml 0.9% normal saline at the last day of the experiment; CSME group, orally received CSME (400 mg/kg BW daily for 5 weeks); APAP group, orally administered with a single dose of APAP (2 g/kg BW); and CSME and APAP group, orally administered with CSME, followed by a single oral dose of APAP. The results of this study revealed that APAP caused a significant increase in serum urea, creatinine concentrations, and malondialdehyde (MDA) concentrations in renal tissues. In addition, APAP caused a significant decrease in superoxide dismutase (SOD) and glutathione peroxidase (GPX) activities in renal tissues compared with the control group. Furthermore, APAP caused marked renal damage as revealed by alterations in histopathological architectures of kidney tissues. APAP resulted in a marked expression of caspase 3 and nuclear factor κB (NFĸβ) within the renal tubules, while caused marked decrease of proliferating cell nuclear antigen (PCNA) immunostaining and transforming growth factor beta 1 (TGFβ 1) expression within the epithelial lining of the renal tubules. However, pre-treatment with CSME returned all biochemical parameters, histopathological changes, and immunohistochemical parameters toward normal levels as the control group. In conclusion, oral administration of CSME protected rats against APAP renal toxicity through its antioxidant, anti-apoptotic, and anti-inflammatory protective mechanisms.
本研究旨在探讨玉米须甲醇提取物（CSME）对乙酰氨基酚（APAP）诱导的肾毒性的保护作用。本研究共纳入 40 只雄性 Wistar 白化大鼠，随机分为 4 组（n=10）：对照组，实验最后一天口服 1.8 ml 0.9%生理盐水；CSME 组，每日口服 CSME（400 mg/kg BW），连续 5 周；APAP 组，口服单次剂量 2 g/kg BW 的 APAP；CSME 和 APAP 组，先口服 CSME，再给予单次口服剂量的 APAP。结果显示，APAP 可导致血清尿素、肌酐浓度和肾组织丙二醛（MDA）浓度显著升高。此外，与对照组相比，APAP 可导致肾组织中超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPX）活性显著降低。此外，APAP 可导致肾组织的组织病理学结构发生改变，从而导致明显的肾损伤。APAP 可导致肾组织中的半胱天冬酶 3 和核因子κB（NFκB）表达明显增加，同时导致肾组织上皮衬里中增殖细胞核抗原（PCNA）免疫染色和转化生长因子β 1（TGFβ 1）表达明显减少。然而，CSME 的预处理可使所有生化参数、组织病理学变化和免疫组织化学参数恢复至对照组的正常水平。综上所述，口服 CSME 通过抗氧化、抗凋亡和抗炎保护机制对 APAP 肾毒性具有保护作用。
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