Sun Fei, Shoffner Adam R, Poss Kenneth D
Regeneration Next, Duke University, Durham, NC, USA.
Department of Cell Biology, Duke University School of Medicine, Durham, NC, USA.
Methods Mol Biol. 2021;2158:71-80. doi: 10.1007/978-1-0716-0668-1_7.
Adult zebrafish possess an elevated cardiac regenerative capacity as compared with adult mammals. In the past two decades, zebrafish have provided a key model system for studying the cellular and molecular mechanisms of innate heart regeneration. The ease of genetic manipulation in zebrafish has enabled the establishment of a genetic ablation injury model in which over 60% of cardiomyocytes can be depleted, eliciting signs of heart failure. After this severe injury, adult zebrafish efficiently regenerate lost cardiomyocytes and reverse heart failure. In this chapter, we describe the methods for inducing genetic cardiomyocyte ablation in adult zebrafish, assessing cardiomyocyte proliferation, and histologically analyzing regeneration after injury.
与成年哺乳动物相比，成年斑马鱼具有更高的心脏再生能力。在过去二十年中，斑马鱼为研究先天性心脏再生的细胞和分子机制提供了关键的模型系统。斑马鱼易于进行基因操作，这使得能够建立一种基因消融损伤模型，其中超过60%的心肌细胞可以被清除，引发心力衰竭的迹象。在这种严重损伤后，成年斑马鱼能够有效地再生失去的心肌细胞并逆转心力衰竭。在本章中，我们描述了在成年斑马鱼中诱导基因心肌细胞消融、评估心肌细胞增殖以及对损伤后再生进行组织学分析的方法。
