Rastoldo Guillaume, El Mahmoudi Nada, Marouane Emna, Pericat David, Watabe Isabelle, Toneto Alain, López-Juárez Alejandra, Chabbert Christian, Tighilet Brahim
Aix Marseille Université-CNRS, Laboratoire de Neurosciences Sensorielles et Cognitives, LNSC UMR 7260. Equipe Physiopathologie et Thérapie des Désordres Vestibulaires, Marseille, Groupe de Recherche Vertige (GDR#2074), France.
Fédération de Recherche Sciences Chimiques Marseille FR 1739, Pôle PRATIM, 13331, Marseille Cedex 03, France.
Prog Neurobiol. 2021 Jan;196:101899. doi: 10.1016/j.pneurobio.2020.101899. Epub 2020 Aug 26.
We previously revealed adult reactive neurogenesis in deafferented vestibular nuclei following unilateral vestibular neurectomy (UVN) in the feline model. We recently replicated the same surgery in a rodent model and aimed to elucidate the origin and fate of newly generated cells following UVN. We used specific markers of cell proliferation, glial reaction, and cell differentiation in the medial vestibular nucleus (MVN) of adult rats. UVN induced an intense cell proliferation and glial reaction with an increase of GFAP-Immunoreactive (Ir), IBA1-Ir and Olig2-Ir cells 3 days after the lesion in the deafferented MVN. Most of the newly generated cells survived after UVN and differentiated into oligodendrocytes, astrocytes, microglial cells and GABAergic neurons. Interestingly, UVN induced a significant increase in a population of cells colocalizing SOX2 and GFAP 3 days after lesion in the deafferented MVN indicating the probable presence of multipotent cells in the vestibular nuclei. The concomitant increase in BrdU- and SOX2-Ir cells with the presence of SOX2 and GFAP colocalization 3 days after UVN in the deafferented MVN may support local mitotic activity of endemic quiescent neural stem cells in the parenchyma of vestibular nuclei.
我们先前在猫模型中进行单侧前庭神经切除术（UVN）后，揭示了去传入前庭核中的成年期反应性神经发生。我们最近在啮齿动物模型中重复了相同的手术，旨在阐明UVN后新生成细胞的起源和命运。我们使用了成年大鼠内侧前庭核（MVN）中细胞增殖、胶质反应和细胞分化的特异性标志物。UVN在损伤后3天，在去传入的MVN中诱导了强烈的细胞增殖和胶质反应，GFAP免疫反应性（Ir）、IBA1-Ir和Olig2-Ir细胞增加。UVN后大多数新生成的细胞存活下来，并分化为少突胶质细胞、星形胶质细胞、小胶质细胞和GABA能神经元。有趣的是，UVN在损伤后3天，在去传入的MVN中诱导了SOX2和GFAP共定位的细胞群体显著增加，表明前庭核中可能存在多能细胞。UVN后3天，去传入的MVN中BrdU和SOX2-Ir细胞的同时增加以及SOX2和GFAP共定位的存在，可能支持前庭核实质中内源性静止神经干细胞的局部有丝分裂活性。
