Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110161, China.
Key Laboratory of Zoonosis of Liaoning Province, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110161, China.
Anim Reprod Sci. 2020 Oct;221:106549. doi: 10.1016/j.anireprosci.2020.106549. Epub 2020 Jul 26.
Cows nearing parturition have a negative energy balance (NEB), which is closely associated with lesser fertility. The NEB results in greater fat mobilisation and production of a large amount of non-esterified fatty acid (NEFA). Prostaglandins (PG), especially prostaglandin E (PGE) and prostaglandin F (PGF), have important functions in regulating reproductive function. There, however, is little known about how the synthesis and release of PG are affected by NEFA. In this study, there was a focus on effects of NEFA on PG secretion as well as relative abundances of mRNA transcript and protein for PG synthetases and PG receptors in bovine endometrial (BEND) cells. Proliferation rate of BEND cells decreased in a concentration-dependent manner as NEFA increased in the media. The concentrations of PGE and PGF in NEFA treatment groups also decreased, while the ratio of PGE/PGF and the relative abundances of proteins and mRNA that regulate PG synthesis and PG receptor mRNA transcripts and protein were greater as the NEFA concentration increased. Collectively, when there were large NEFA concentrations in the medium, there was a lesser release of PGE and PGF, however, there was a greater ratio of PGE/PGF and relative abundances of mRNA transcripts and protein for PG synthetases and PG receptors in BEND cells, which changed the internal milieu and physiological function of the uterus with possible effects on fertility after calving. These findings provide important information that will help for further investigation of associations between NEB and fertility in dairy cows during the non-lactation to lactation-transition period.
临近分娩的奶牛处于负能平衡（NEB）状态，这与较低的繁殖力密切相关。NEB 导致脂肪动员增加，并产生大量非酯化脂肪酸（NEFA）。前列腺素（PG），尤其是前列腺素 E（PGE）和前列腺素 F（PGF），在调节生殖功能方面具有重要作用。然而，人们对 NEFA 如何影响 PG 的合成和释放知之甚少。在这项研究中，我们关注 NEFA 对 PG 分泌以及牛子宫内膜（BEND）细胞中 PG 合成酶和 PG 受体的 mRNA 转录本和蛋白相对丰度的影响。随着培养基中 NEFA 浓度的增加，BEND 细胞的增殖率呈浓度依赖性下降。PGF 处理组的 PGE 和 PGF 浓度也降低，而 PGE/PGF 比值以及调节 PG 合成和 PG 受体 mRNA 转录本和蛋白的 PG 合成酶和 PG 受体的蛋白和 mRNA 相对丰度随着 NEFA 浓度的增加而增加。总的来说，当培养基中存在大量的 NEFA 时，PGE 和 PGF 的释放减少，但 BEND 细胞中 PGE/PGF 比值以及 PG 合成酶和 PG 受体的 mRNA 转录本和蛋白的相对丰度增加，这改变了子宫的内部环境和生理功能，可能对产后的繁殖力产生影响。这些发现提供了重要信息，有助于进一步研究奶牛在非泌乳期到泌乳过渡期期间 NEB 与繁殖力之间的关系。
