Kimmins Sarah, Lim Hai Choo, Parent Julie, Fortier Michel A, MacLaren Leslie A
Department of Plant and Animal Sciences, Nova Scotia Agricultural College, Box 550, Truro, NS, Canada B2N 5E3.
Domest Anim Endocrinol. 2003 Aug;25(2):141-54. doi: 10.1016/s0739-7240(03)00015-8.
In cattle, endometrial expression of integrin alphavbeta3 is reduced on day 16 of the estrous cycle, coinciding with the critical period during which the decision is made to initiate luteolysis or continue with pregnancy. The objective of these experiments was to examine the relationship between estrogen and progesterone treatments, endometrial integrin alphavbeta3 expression, and prostaglandin F2alpha (PGF2alpha) and E2 (PGE2) production. Epithelial and stromal cells from intercaruncular (ICAR) and caruncular (CAR) bovine endometrium were treated with 17beta-estradiol (0.1 and 1.0 nM) and/or progesterone (1.0 and 10 nM) in a manner designed to mimic the steroid fluctuations of the estrous cycle. All cell types expressed estrogen receptor and progesterone receptor mRNA and protein. Intercaruncular stromal cells were the most responsive to steroidal regulation. Estrogen suppressed expression of integrin subunit beta3 mRNA in ICAR stromal cells (P< or =0.05). Progesterone and estrogen + progesterone treated cells did not differ in beta3 expression from controls (P> or =0.05). Steroid treatment did not affect PGF2alpha production in any cell type (P> or =0.05), however, estrogen decreased PGE2 production in all cells except CAR stroma (P< or =0.05). The results indicate that in bovine endometrium expression of integrin alphavbeta3 and production of PGE2 is influenced by estrogen.
在牛中，发情周期第16天时子宫内膜中整合素αvβ3的表达降低，这与决定启动黄体溶解或继续妊娠的关键时期相吻合。这些实验的目的是研究雌激素和孕酮处理、子宫内膜整合素αvβ3表达以及前列腺素F2α（PGF2α）和E2（PGE2）产生之间的关系。以模拟发情周期类固醇波动的方式，用17β-雌二醇（0.1和1.0 nM）和/或孕酮（1.0和10 nM）处理来自牛子宫肉阜间（ICAR）和肉阜（CAR）子宫内膜的上皮细胞和基质细胞。所有细胞类型均表达雌激素受体和孕酮受体mRNA及蛋白。子宫肉阜间基质细胞对类固醇调节反应最为敏感。雌激素抑制ICAR基质细胞中整合素亚基β3 mRNA的表达（P≤0.05）。孕酮以及雌激素+孕酮处理的细胞在β3表达上与对照无差异（P≥0.05）。类固醇处理对任何细胞类型的PGF2α产生均无影响（P≥0.05），然而，雌激素降低了除CAR基质外所有细胞中的PGE2产生（P≤0.05）。结果表明，在牛子宫内膜中，整合素αvβ3的表达和PGE2的产生受雌激素影响。
