National Association of Rasa Aragonesa Breeders (ANGRA), Zuera, 50800, Spain.
Department of Animal Pathology, Universidad de Zaragoza, Zaragoza, 50013, Spain.
Anim Reprod Sci. 2020 Oct;221:106551. doi: 10.1016/j.anireprosci.2020.106551. Epub 2020 Jul 22.
There has been development of an antiretrograde flow device (DARIO), for sheep cervical artificial insemination (CAI). There, however, needs to be optimization of sperm volume and concentration of insemination doses when the DARIO is used for CAI. Objectives were to compare fertility rates (proportion of ewes lambing as a result of CAI) when there was use of the DARIO for CAI: two sperm volumes containing equal numbers of spermatozoa: 0.25 mL of 1,600 × 10 spermatozoa/mL and 0.50 mL of 800 × 10 spermatozoa/mL (Test 1 group), and two sperm volumes with a different number of spermatozoa/AI dose: 0.25 mL and 0.50 mL of 1,600 × 10 spermatozoa/mL (Test 2 group). There were 335 ewes from seven farms assigned to 60 batches (equally divided into a Control and Test 1 group). For the Test 2 group, 462 ewes from nine farms were assigned to 88 batches (equally proportioned into Control group and Test 2 groups). For the Test 1 group, proportion of ewes lambing as a result of CAI were 0.701 ± 0.2679 and 0.595 ± 0.2393 for the Control and Test 1 groups, respectively (P = 0.163). For the Test 2 group, proportions of ewes lambing were 0.550 ± 0.2598 and 0.658 ± 0.2412 for the Control and Test 2 group, respectively (P = 0.041). An inclusion of a larger number of spermatozoa per insemination in a 0.50 mL dose volume resulted improved proportion of ewes lambing as a result of CAI when there was used of the DARIO.
已经开发出一种用于绵羊宫颈人工授精（CAI）的逆流装置（DARIO）。然而，当使用 DARIO 进行 CAI 时，需要优化精子体积和授精剂量的浓度。目的是比较使用 DARIO 进行 CAI 时的受精率（CAI 导致母羊产羔的比例）：两种包含等量精子的精子体积：0.25ml 为 1600×10 个精子/ml 和 0.50ml 为 800×10 个精子/ml（测试 1 组），以及两种精子体积与不同的精子数/AI 剂量：0.25ml 和 0.50ml 为 1600×10 个精子/ml（测试 2 组）。共有来自七个农场的 335 只母羊被分配到 60 个批次（平均分为对照组和测试 1 组）。对于测试 2 组，来自九个农场的 462 只母羊被分配到 88 个批次（平均分为对照组和测试 2 组）。对于测试 1 组，CAI 产羔的母羊比例分别为对照组和测试 1 组的 0.701±0.2679 和 0.595±0.2393（P=0.163）。对于测试 2 组，对照组和测试 2 组的产羔比例分别为 0.550±0.2598 和 0.658±0.2412（P=0.041）。在 0.50ml 剂量体积中包含更多的精子数量，当使用 DARIO 时，CAI 导致母羊产羔的比例会提高。
