College of Veterinary Medicine, Yunnan Agricultural University, Kunming City, Yunnan Province, China.
Yunnan Animal Science and Veterinary Institute, Jindian, Panlong County, Kunming City, Yunnan Province, China.
Sci Rep. 2021 Jul 30;11(1):15562. doi: 10.1038/s41598-021-95138-9.
Sperm motility is an index tightly associated with male fertility. A close relationship between seminal plasma and sperm motility has been confirmed. This study was to assess the protein and metabolite profiles of seminal plasma obtained from adult goats with high or low sperm motility using the proteomic and metabolomic strategies. In total, 2098 proteins were found. 449 differentially abundant proteins (DAPs) were identified, and 175 DAPs were enriched in the high motility group. The obtained DAPs primarily exist in cytoplasma and extra-cellular portion. The Gene Ontology enrichment analysis demonstrated the main functional roles of these DAPs in regulating biological process, metabolic process of organic substances, cellular-metabolic process, primary-metabolic process, metabolic process of nitrogen compounds, etc. Additionally, the Kyoto-Encyclopedia of Genes and Genomes (KEGG) analysis revealed that these DAPs were primarily involved in phosphatidylinositol signaling system, salivary secretion, proteasome, apoptosis, mitophagy-animal, etc. Aided by the parallel reaction monitoring technology, the abundance changing pattern of 19 selected DAPs was consistent with that of the corresponding proteins obtained by TMT. A total of 4603 metabolites were identified in seminal plasma. 1857 differential metabolites were found between the high motility group and the low motility group, and 999 metabolites were up-regulated in the high motility group. The KEGG analysis demonstrated the primary involvement of the differential metabolites in metabolic and synthetic activities. In conclusion, we first established the proteome and metabolome databank of goat seminal plasma, detecting some proteins and metabolites which may affect sperm motility. This study will be valuable for understanding mechanisms leading to poor sperm motility.
精子活力是与男性生育力密切相关的指标。已经证实精浆与精子活力之间存在密切关系。本研究采用蛋白质组学和代谢组学策略，评估了来自精子活力高或低的成年山羊的精浆中的蛋白质和代谢物图谱。共发现 2098 种蛋白质。鉴定出 449 种差异丰度蛋白（DAP），其中 175 种 DAP 在高活力组中富集。获得的 DAP 主要存在于细胞质和细胞外部分。基因本体论富集分析表明，这些 DAP 的主要功能作用是调节生物过程、有机物质的代谢过程、细胞代谢过程、初级代谢过程、含氮化合物的代谢过程等。此外，京都基因与基因组百科全书（KEGG）分析表明，这些 DAP 主要参与磷脂酰肌醇信号系统、唾液分泌、蛋白酶体、细胞凋亡、动物线粒体自噬等。通过平行反应监测技术，19 种选定的 DAP 的丰度变化模式与 TMT 获得的相应蛋白质一致。在精浆中鉴定出 4603 种代谢物。在高活力组和低活力组之间发现 1857 种差异代谢物，其中 999 种在高活力组中上调。KEGG 分析表明，差异代谢物主要参与代谢和合成活动。总之，我们首次建立了山羊精浆的蛋白质组和代谢组数据库，检测到一些可能影响精子活力的蛋白质和代谢物。本研究将有助于理解导致精子活力差的机制。
