Department of Microbiology, Medical Research Institute, Alexandria University, Egypt.
Department of Biomedical Informatics and Medical Statistics, Medical Research Institute, Alexandria University, Egypt.
Diagn Microbiol Infect Dis. 2020 Nov;98(3):115139. doi: 10.1016/j.diagmicrobio.2020.115139. Epub 2020 Jul 12.
This study compared genotyping of Klebsiella pneumoniae isolates by 2 molecular methods. Genotyping of 50 multidrug-resistant (MDR) and 10 non-MDR K. pneumoniae subsp. pneumoniae isolates from 2 hospitals was done using multiple locus variable number tandem repeat analysis (MLVA) and capsular typing by wzi gene sequencing. Genotyping of the isolates by the 2 methods showed 100% typeability. Agreement on clustering of the isolates by the 2 methods was 82.6%. Typing by MLVA, however, was more discriminatory (97%) than by wzi gene sequencing (92%). All the 23 K. pneumoniae subsp. pneumoniae isolates randomly selected for wzi gene sequencing showed sequence identity to previously published wzi sequences, which enabled prediction of the K-types of 16 of them. The 2 methods revealed the relatedness of (8/15) isolates from 1 of the 2 hospitals. MLVA may be considered a cheaper and more discriminatory molecular typing method suitable for genotyping of K. pneumoniae isolates in developing countries.
本研究比较了两种分子方法对肺炎克雷伯菌分离株的基因分型。对来自两家医院的 50 株多药耐药（MDR）和 10 株非 MDR 肺炎克雷伯菌亚种肺炎克雷伯菌进行了多位点可变数串联重复分析（MLVA）和 wzi 基因测序的荚膜分型。两种方法对分离株的基因分型均具有 100%的可分型性。两种方法聚类分离株的一致性为 82.6%。然而，MLVA 分型比 wzi 基因测序（92%）更具区分性（97%）。随机选择用于 wzi 基因测序的 23 株肺炎克雷伯菌亚种肺炎克雷伯菌的所有分离株与先前发表的 wzi 序列具有相同的序列同一性，这使得能够预测其中 16 株的 K 型。两种方法均揭示了来自其中一家医院的 15 株分离株中的（8/15）的相关性。MLVA 可被认为是一种更具经济性和区分性的分子分型方法，适用于发展中国家肺炎克雷伯菌分离株的基因分型。
