成团泛菌7460的脂多糖：O-特异性多糖、脂质A结构及生物活性

Lipopolysaccharide of Pantoea agglomerans 7460: O-specific polysaccharide and lipid A structures and biological activity.

作者信息

Zdorovenko Evelina L, Kadykova Alexandra A, Shashkov Alexander S, Varbanets Liudmyla D, Bulyhina Tetiana V, Knirel Yuriy A

机构信息

N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Leninsky Prospekt 47, 119991, Moscow, Russia.

出版信息

Carbohydr Res. 2020 Oct;496:108132. doi: 10.1016/j.carres.2020.108132. Epub 2020 Aug 15.

DOI:10.1016/j.carres.2020.108132

PMID:32861900

Abstract

Lipopolysaccharide (LPS) was isolated from Pantoea agglomerans 7460 cells by phenol-water extraction. Mild acid degradation allowed to separate OPS and lipid A. Lipid A was analyzed by negative-ion mode ESI MS and found to consist mainly of hexaacylated derivative containing biphosphorylated GlcN disaccharide, four 14:0 (3-OH), 18:0 and 12:0 fatty acids. The structure of the O-specific polysaccharide was established by chemical, NMR and computational methods: The LPS of Р. agglomerans 7460 showed low level of toxicity and pyrogenicity to compare with LPS of E. coli O55:B5 and pyrogenal, respectively. The ability of the modified (succinylated) LPS, which have lost its toxicity, to block the toxic effects of native LPS has been shown.

摘要

通过苯酚-水萃取法从成团泛菌7460细胞中分离出脂多糖（LPS）。轻度酸降解可分离出O-多糖（OPS）和脂质A。脂质A通过负离子模式电喷雾电离质谱（ESI MS）进行分析，发现其主要由含有双磷酸化葡糖胺二糖、四个14:0（3-OH）、18:0和12:0脂肪酸的六酰化衍生物组成。通过化学、核磁共振（NMR）和计算方法确定了O-特异性多糖的结构：与大肠杆菌O55:B5的LPS和热原质相比，成团泛菌7460的LPS毒性和致热原性水平较低。已证明失去毒性的修饰（琥珀酰化）LPS能够阻断天然LPS的毒性作用。