基于β-环糊精修饰碳纳米粒子的主客体识别测定牛奶中的三聚氰胺。
Determination of melamine in milk based on β-cyclodextrin modified carbon nanoparticles via host-guest recognition.
机构信息
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China; Guangxi Key Laboratory of Petrochemical Resource Processing and Process Intensification Technology, School of Chemistry and Chemical Engineering, Guangxi University, Nanning 530004, China.
出版信息
Food Chem. 2021 Feb 15;338:127769. doi: 10.1016/j.foodchem.2020.127769. Epub 2020 Aug 5.
Illegal addition of melamine (MEL) to milk has caused serious food safety accident. It is urgent to develop a highly sensitive method for detecting MEL in milk. β-Cyclodextrin with inner hydrophobic and outer hydrophilic cavities have been widely used in smart sensors design. In this study, an "ON-OFF-ON" sensor for MEL detection was constructed based on β-cyclodextrin modified carbon nanoparticles (β-CD-CNPs). The sensor is switched "OFF" when Fe interacts with β-CD-CNPs and switched "ON" when MEL replaces Fe. Fluorescence recovery of β-CD-CNPs exhibits good linear correlations with MEL concentration ranging from 10.00 ng/mL ~ 180.00 ng/mL and 180.00 ~ 1000.00 ng/mL, the detection limit is 6.82 ng/mL. The sensor was applied to analysis melamine in milk samples with recovery between 94.80% ~ 102.05%, and RSD bellow 12.61%. The results show that this method can meet the requirements of real sample analysis.
三聚氰胺（MEL）非法添加到牛奶中已造成严重的食品安全事故。因此，迫切需要开发一种高灵敏度的方法来检测牛奶中的 MEL。具有内疏水和外亲水空腔的β-环糊精已广泛应用于智能传感器设计中。在这项研究中，基于β-环糊精修饰的碳纳米粒子（β-CD-CNPs）构建了用于检测 MEL 的“开-关-开”传感器。当 Fe 与β-CD-CNPs 相互作用时，传感器处于“关”状态，当 MEL 取代 Fe 时，传感器处于“开”状态。β-CD-CNPs 的荧光恢复与 MEL 浓度在 10.00ng/mL180.00ng/mL 和 180.00ng/mL1000.00ng/mL 范围内呈良好的线性相关，检测限为 6.82ng/mL。该传感器用于分析牛奶样品中的三聚氰胺，回收率在 94.80%~102.05%之间，相对标准偏差（RSD）低于 12.61%。结果表明，该方法能够满足实际样品分析的要求。
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