Department of Biology, Faculty of Science, Taif University , Taif, Kingdom of Saudi Arabia.
, Plant Genetic Transformation Department, Agricultural Genetic Engineering Research Institute (AGERI), Agricultural Research Center (ARC) , Giza, Egypt.
GM Crops Food. 2021 Jan 1;12(1):47-56. doi: 10.1080/21645698.2020.1809318.
The sugarcane ( X ) is one of the most important crops used to produce sugar and raw material for biofuel in the world. One of the main causes for sucrose content and yield losses is the attack by insect. In this investigation, gene was introduced into sugarcane variety GT54-9(C9) using the transformation method for transgenic sugarcane production presenting insect-resistance. The strain GV1303 including pART vector was used for the production of transformed sugarcane. The gene were successfully used to produce transgenic plants used for the improvement of both agronomic efficiency and product quality by acquiring insect resistance. PCR and Southern hybridization techniques were used to confirm the gene incorporation into sugarcane genome. Transformation percentage was 22.2% using PCR analysis with specific primers for and (Neomycin phosphotransferase) genes. The expression of gene was determined using reverse transcriptase polymerase chain reaction (RT-PCR), QuickStix test, and insect bioassays. Bioassays for transformed sugarcane plants showed high level of toxicity to giving 100% mortality of the larvae. Sugarcane insect resistance was improved significantly by using gene transformation.
甘蔗（X）是世界上用于生产糖和生物燃料原料的最重要作物之一。导致甘蔗蔗糖含量和产量损失的主要原因之一是昆虫的侵害。在这项研究中，利用转化方法将基因导入甘蔗品种 GT54-9（C9），以生产具有抗虫性的转基因甘蔗。使用包括 pART 载体的菌株 GV1303 生产转化甘蔗。成功地使用 基因生产转基因植物，通过获得抗虫性来提高农艺效率和产品质量。利用针对 和 （新霉素磷酸转移酶）基因的特异性引物进行 PCR 和 Southern 杂交技术证实了 基因整合到甘蔗基因组中。使用针对 和 （新霉素磷酸转移酶）基因的特异性引物进行 PCR 分析，转化百分率为 22.2%。使用逆转录聚合酶链反应（RT-PCR）、QuickStix 测试和昆虫生物测定确定 基因的表达。对转化甘蔗植物的生物测定显示对 具有高毒性，导致幼虫死亡率达到 100%。通过使用 基因转化，显著提高了甘蔗的抗虫性。
