Mohteshamuddin Khaja, Hamdan Layaly, AlKaabi Abdulla B, Barigye Robert
Veterinary Medicine Department, College of Food and Agriculture, United Arab Emirates University, PO Box 15551, Al Ain, United Arab Emirates.
Veterinary Medicine Department, College of Food and Agriculture, United Arab Emirates University, PO Box 15551, Al Ain, United Arab Emirates.
Vet Parasitol Reg Stud Reports. 2020 Jul;21:100435. doi: 10.1016/j.vprsr.2020.100435. Epub 2020 Jul 17.
Faecal specimens from 36 scouring neonatal calves from two dairy farms located in the Al Ain region of the UAE were screened with pathogen-specific antigen ELISA for Cryptosporidium parvum, Escherichia coli K99, rotavirus, and coronavirus. Additionally, faecal smears were stained with modified-acid-fast for Cryptosporidium oocysts, and the VITEK 2 system plus Gram's stain used to identify bacteria isolated from the faecal samples. Farm management practices were also evaluated during a farm visit. Of the 36 calves, 29, 13, 5, and 6 were positive for C. parvum, E. coli K99, bovine coronavirus, and rotavirus antigens respectively, while 27 were positive for Cryptosporidium oocysts. In various combinations, mixed infections were detected in 20/36 calves. This is the first report of C. parvum, E. coli K99, Salmonella spp., rotavirus, and coronavirus in ≤14-days-old scouring neonatal dairy calves from the UAE. Molecular characterization of these pathogens and nationwide epidemiological calf scour studies are recommended.
对来自阿联酋艾因地区两个奶牛场的36头腹泻新生犊牛的粪便样本，采用病原体特异性抗原ELISA法检测微小隐孢子虫、大肠杆菌K99、轮状病毒和冠状病毒。此外，粪便涂片用改良抗酸染色法检测隐孢子虫卵囊，并用VITEK 2系统加革兰氏染色法鉴定从粪便样本中分离出的细菌。在农场访问期间还评估了农场管理措施。36头犊牛中，分别有29头、13头、5头和6头微小隐孢子虫、大肠杆菌K99、牛冠状病毒和轮状病毒抗原检测呈阳性，27头隐孢子虫卵囊检测呈阳性。在20/36头犊牛中检测到各种组合的混合感染。这是阿联酋≤14日龄腹泻新生奶牛犊中微小隐孢子虫、大肠杆菌K99、沙门氏菌属、轮状病毒和冠状病毒的首次报告。建议对这些病原体进行分子特征分析，并开展全国范围的犊牛腹泻流行病学研究。
