CMH Institute of Medical Sciences Bahawalpur, Bahawalpur, Pakistan/Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, University of Karachi, Karachi, Pakistan.
Pak J Pharm Sci. 2020 Mar;33(2(Supplementary)):779-786.
This study was aimed to investigate the antioxidant effects of Syzygium cumini (S. cumini) seeds extract along with wound healing score in correlation to glutathione and blood glucose levels in diabetic rats. Sprague-Dawley rats were divided into 8 groups (n=6) and diabetes was induced by a single intraperitoneal injection of streptozotocin (40 mg kg-1 body weight). An alcoholic extract of S. cumini was administered to punch plier induced wounded rats and changes in serum glutathione levels, wound healing score and blood glucose levels were examined. Laser treatment of 500 mW for 5 minutes was given once a day for 12 days at 810 and 630 nm respectively. Statistical analysis was performed using one way ANOVA. A p-value <0.05 was considered significant and p-value <0.001 was considered highly significant. There was significant increase in glutathione levels and wound healing score when S. cumini extract was administered oral and topical along with topical laser therapy. There was significant reduction in the blood glucose levels upon administration of S. cumini extract in diabetic rats. This study concludes that there was a positive relation between the glutathione levels and wound healing score, since there was increase in wound healing score with the increase in the glutathione levels.
本研究旨在探讨 Syzygium cumini（S. cumini）种子提取物的抗氧化作用，以及与谷胱甘肽和血糖水平相关的伤口愈合评分在糖尿病大鼠中的相关性。将 Sprague-Dawley 大鼠分为 8 组（n=6），通过单次腹腔注射链脲佐菌素（40 mg kg-1 体重）诱导糖尿病。向打孔钳诱导的创伤大鼠给予 S. cumini 的酒精提取物，并检查血清谷胱甘肽水平、伤口愈合评分和血糖水平的变化。以 500 mW 的激光分别在 810 和 630nm 下每天治疗 5 分钟，共 12 天。使用单因素方差分析进行统计分析。p 值<0.05 被认为具有统计学意义，p 值<0.001 被认为具有高度统计学意义。当 S. cumini 提取物口服和局部联合局部激光治疗时，谷胱甘肽水平和伤口愈合评分显著增加。在糖尿病大鼠中给予 S. cumini 提取物后，血糖水平显著降低。本研究得出结论，谷胱甘肽水平与伤口愈合评分之间存在正相关关系，因为随着谷胱甘肽水平的升高，伤口愈合评分也随之升高。
