Sweeney Robin E, Nguyen Vina, Alouidor Benjamin, Budiman Elizabeth, Wong Raymond K, Yoon Jeong-Yeol
Department of Biomedical Engineering, The University of Arizona, Tucson, AZ 85721, USA; Unchained Labs, Pleasanton, CA, USA.
Perfusion Sciences Graduate Program, Department of Medical Pharmacology, The University of Arizona College of Medicine, Tucson, AZ 85721, USA; Pacific Life Lines, San Carlos, CA, USA.
IEEE Sens J. 2019 Jul 1;19(13):4743-4751. doi: 10.1109/jsen.2019.2902065. Epub 2019 Feb 27.
Monitoring blood coagulation in response to an anticoagulant (heparin) and its reversal agent (protamine) is essential during and after surgery, especially with cardiopulmonary bypass (CPB). A current clinical standard is the use of activated clotting time (ACT), where the mechanical movement of a plunger through a whole blood-filled channel is monitored to evaluate the endpoint time of coagulation. As a rapid, simple, low-volume, and cost-effective alternative, we have developed a paper microfluidic assay and Raspberry Pi-based device with the aim of quantifying the extent of blood coagulation in response to varying doses of heparin and protamine. The flow rate of blood through the paper microfluidic channel is automatically monitored using Python-coded edge detection algorithm. For each set of assay, 8 L of fresh human whole blood (untreated and undiluted) from human subjects is loaded onto each of 8 sample pads, which have been preloaded with varying amounts of heparin or protamine. Total assay time is 3-5 minutes including the time for sample loading and incubation.
在手术期间及术后，尤其是在体外循环（CPB）过程中，监测血液对抗凝剂（肝素）及其逆转剂（鱼精蛋白）的凝血反应至关重要。当前的临床标准是使用活化凝血时间（ACT），通过监测柱塞在充满全血的通道中的机械运动来评估凝血终点时间。作为一种快速、简单、低体积且经济高效的替代方法，我们开发了一种纸质微流控分析方法以及基于树莓派的设备，旨在量化血液对不同剂量肝素和鱼精蛋白的凝血程度。使用Python编码的边缘检测算法自动监测血液通过纸质微流控通道的流速。对于每组分析，将来自人类受试者的8微升新鲜人全血（未处理且未稀释）加载到8个样品垫上，每个样品垫已预先加载了不同量的肝素或鱼精蛋白。总分析时间为3至5分钟，包括样品加载和孵育时间。
