Silvani Yulia, Lovita Agnestia Naning Dian, Maharani Afniari, Wiyasa I Wayan Arsana, Sujuti Hidayat, Ratnawati Retty, Raras Tri Yudani Mardining
Midwifery Department, Faculty of Medicine, University of Brawijaya, Malang, Indonesia.
Department of Obstetrics and Gynecology, Dr. Saiful Anwar General Hospital, Malang, Indonesia.
J Family Reprod Health. 2020 Mar;14(1):38-44.
This study aimed to determine the effect of Extra Virgin Olive Oil (EVOO) on vasodilator enzyme by repairing angiogenic function in rat model of preeclampsia. This research consisted of five groups; negative control (normal pregnant rats) group, positive control (preeclampsia rat model) group, preeclampsia rat model groups given EVOO in 3 different doses (0.5 ml/day, 1 ml/day, and 2 ml/day, respectively). Blood pressure measurements were carried out on day 12, 15, and 19 of pregnancy. After the rats were sacrificed, the placentas were collected to determine endothelial Nitric Oxide Synthase (eNOS) level of maternal plasma to determine soluble Fms-like Tyrosine Kinase 1 (sFlt-1) and Vascular Endothelial Growth Factor (VEGF) level. There were significant higher sFlt-1 level (p < 0.001), lower VEGF level (p = 0.009), and lower eNOS level (p = 0.034) between negative and positive control groups. After EVOO administration, sFlt-1 level was lower in dose 1 and 2 groups but higher in dose 3 group in accordance with VEGF and eNOS levels that were increasing both in dose 1 and dose 2 groups but decreasing in dose 3. There were significant differences between positive control and dose 1 (p = 0.015) and dose 2 (p = 0.001) in sFlt-1 level. None of all dose groups were statistically different with positive control group in VEGF level (dose 1 p = 0.601; dose 2 p = 0.297; dose 3 p = 0.805). eNOS levels of all dose groups were statistically different from that of the positive control group (dose 1 p = 0.014; dose 2 p = 0.001; dose 3 p = 0.024). Administration of EVOO modulates eNOS as vasodilator enzyme by repairing the angiogenic function indicated by decreased sFlt-1 level and increased VEGF in rat model of preeclampsia.
本研究旨在通过修复子痫前期大鼠模型的血管生成功能，确定特级初榨橄榄油（EVOO）对血管舒张酶的影响。本研究分为五组：阴性对照组（正常妊娠大鼠）、阳性对照组（子痫前期大鼠模型）、子痫前期大鼠模型给予三种不同剂量EVOO的组（分别为0.5毫升/天、1毫升/天和2毫升/天）。在妊娠第12、15和19天进行血压测量。大鼠处死后，收集胎盘以测定母体血浆中内皮型一氧化氮合酶（eNOS）水平，以确定可溶性Fms样酪氨酸激酶1（sFlt-1）和血管内皮生长因子（VEGF）水平。阴性对照组和阳性对照组之间，sFlt-1水平显著更高（p < 0.001），VEGF水平更低（p = 0.009），eNOS水平更低（p = 0.034）。给予EVOO后，剂量1组和剂量2组的sFlt-1水平较低，而剂量3组较高，这与剂量1组和剂量2组中VEGF和eNOS水平升高但剂量3组降低一致。阳性对照组与剂量1组（p = 0.015）和剂量2组（p = 0.001）的sFlt-1水平存在显著差异。所有剂量组的VEGF水平与阳性对照组均无统计学差异（剂量1组p = 0.601；剂量2组p = 0.297；剂量3组p = 0.805）。所有剂量组的eNOS水平与阳性对照组均有统计学差异（剂量1组p = 0.014；剂量2组p = 0.001；剂量3组p = 0.024）。在子痫前期大鼠模型中，给予EVOO通过修复血管生成功能来调节作为血管舒张酶的eNOS，表现为sFlt-1水平降低和VEGF升高。
