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精氨酸加压素在下丘脑大细胞神经分泌细胞中的过表达及其与糖尿病肾病发生发展的潜在关系。

Over-expression of arginine vasopressin in magnocellular neurosecretory cells of hypothalamus and its potential relationship with development of diabetic nephropathy.

作者信息

Li Xianhua, Gao Zhaoli, Xu Ling, Li Baoying, Gao Haiqing

机构信息

Department of Nephrology, Qi Lu Hospital of Shandong University, Jinan, China.

Department of Geriatrics, Qi Lu Hospital of Shandong University, Jinan, China.

出版信息

Arch Med Sci. 2020 Jan 19;16(5):1130-1139. doi: 10.5114/aoms.2020.92402. eCollection 2020.

DOI:10.5114/aoms.2020.92402
PMID:32864002
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7444698/
Abstract

INTRODUCTION

We aimed to assess our hypothesis that the expression changes of arginine vasopressin (AVP) in the magnocellular neurosecretory cells (MNCs) of hypothalamus and V2 receptor for AVP (RVP) in kidney may contribute to the pathogenesis of diabetic nephropathy (DN).

MATERIAL AND METHODS

Twenty-five male Wistar rats were randomly assigned to the control group and the diabetes mellitus (DM) group. Periodic acid-Schiff (PAS) staining and electron microscopy were used for morphological studies. Immunohistochemical staining for glial fibrillary acidic protein (GFAP) is standard for visualization of reactive astrocytes in the hypothalamus. Hypothalamus was used for immunofluorescence of AVP. Kidney was used for immunohistochemical staining of RVP. Quantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) was used for quantitative determinations of AVP mRNA in hypothalamus and RVP mRNA in kidney. Western blot was used to measure the protein expression of AVP in hypothalamus and RVP in kidney.

RESULTS

Morphological studies showed abnormalities in kidney and hypothalamus in the DM group. The number of neurons and the gray value of astrocytes in hypothalamus in the DM group were markedly decreased. The expression level of AVP in hypothalamus and the expression level of RVP in kidney of DM rats were significantly increased. The positive correlations between the proteinuria and expression (mRNA and protein) of AVP, proteinuria and expression (mRNA and protein) of RVP, and the expression of AVP and RVP levels were found.

CONCLUSIONS

AVP was upregulated in the MNCs of hypothalamus and RVP was upregulated in kidney in streptozotocin-induced DM rats, indicating their potential roles in the development of DN.

摘要

引言

我们旨在评估我们的假设，即下丘脑大细胞神经分泌细胞（MNCs）中精氨酸加压素（AVP）的表达变化以及肾脏中AVP的V2受体（RVP）可能参与糖尿病肾病（DN）的发病机制。

材料与方法

25只雄性Wistar大鼠随机分为对照组和糖尿病（DM）组。采用过碘酸希夫（PAS）染色和电子显微镜进行形态学研究。胶质纤维酸性蛋白（GFAP）免疫组织化学染色是下丘脑反应性星形胶质细胞可视化的标准方法。下丘脑用于AVP的免疫荧光检测。肾脏用于RVP的免疫组织化学染色。采用定量实时逆转录聚合酶链反应（qRT-PCR）定量测定下丘脑AVP mRNA和肾脏RVP mRNA。蛋白质印迹法用于检测下丘脑AVP和肾脏RVP的蛋白表达。

结果

形态学研究显示DM组肾脏和下丘脑存在异常。DM组下丘脑神经元数量和星形胶质细胞灰度值明显降低。DM大鼠下丘脑AVP表达水平和肾脏RVP表达水平显著升高。发现蛋白尿与AVP表达（mRNA和蛋白）、蛋白尿与RVP表达（mRNA和蛋白）以及AVP和RVP水平表达之间呈正相关。

结论

在链脲佐菌素诱导的DM大鼠中，下丘脑MNCs中AVP上调，肾脏中RVP上调，表明它们在DN发生发展中具有潜在作用。

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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/7444698/f2dd807181fc/AMS-16-5-39629-g001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/7444698/ea3ebc33c3dc/AMS-16-5-39629-g005.jpg

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