Yadav Sweta K, Khatri Kusum, Rathore Mangal S, Jha Bhavanath
Academy of Scientific and Innovative Research (AcSIR), Ghaziabad, India.
Division of Applied Phycology and Biotechnology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), Bhavnagar, India.
DNA Cell Biol. 2024 Dec;43(12):e630-e644. doi: 10.1089/dna.2020.5479. Epub 2020 Aug 31.
Cytochrome bf complex is a thylakoid membrane-localized protein and catalyses the transfer of electrons from plastoquinol to plastocyanin in photosynthetic electron transport chain. In the present study, () gene from (a red seaweed) was overexpressed in tobacco. A 935 base pair (bp) long cDNA contained an open reading frame of 648 bp encoding a protein of 215 amino acids with an expected isoelectric point of 8.67 and a molecular mass of 24.37 kDa. The gene was overexpressed in tobacco under control of CaMV35S promoter. The transgenic tobacco had higher electron transfer rate and photosynthetic yield over wild-type and vector control tobacco. The tobacco also exhibited significantly higher photosynthetic gas exchange (P) and improved water use efficiency. The transgenic plants had higher ratio of P and intercellular CO. The transgenic tobacco showed higher estimates of photosystem II quantum yield, higher activity of the water-splitting complex, PSII photochemistry, and photochemical quenching. The basal quantum yield of nonphotochemical processes in PSII was recorded lower in tobacco. Transgenic tobacco contained higher contents of carotenoids and total chlorophyll and also had better ratios of chlorophyll a and b, and carotenoids and total chlorophyll contents hence improved photosynthetic efficiency and production of sugar and starch. The transgenic plants performed superior under control and greenhouse conditions. To the best of our knowledge through literature survey, this is the first report on characterization of gene from for enhanced photosynthetic efficiency and growth in tobacco.
细胞色素bf复合物是一种定位于类囊体膜的蛋白质，在光合电子传递链中催化电子从质体醌向质体蓝素的转移。在本研究中，来自（一种红藻）的（）基因在烟草中过表达。一个935碱基对（bp）长的cDNA包含一个648 bp的开放阅读框，编码一个215个氨基酸的蛋白质，预期等电点为8.67，分子量为24.37 kDa。该基因在CaMV35S启动子的控制下在烟草中过表达。转基因烟草比野生型和载体对照烟草具有更高的电子传递速率和光合产量。该烟草还表现出显著更高的光合气体交换（P）和提高的水分利用效率。转基因植株具有更高的P与胞间CO2比值。该转基因烟草显示出更高的光系统II量子产率估计值、更高的水裂解复合物活性、PSII光化学和光化学猝灭。PSII中非光化学过程的基础量子产率在该烟草中记录较低。转基因烟草含有更高含量的类胡萝卜素和总叶绿素，并且叶绿素a与b以及类胡萝卜素与总叶绿素含量的比例也更好，因此提高了光合效率以及糖和淀粉的产量。该转基因植株在对照和温室条件下表现优异。据我们通过文献调查所知，这是关于来自用于提高烟草光合效率和生长的基因特性的首次报道。
