Yadav Sweta K, Khatri Kusum, Rathore Mangal S, Jha Bhavanath
Academy of Scientific and Innovative Research, CSIR, New Delhi, India.
Division of Biotechnology and Phycology, CSIR-Central Salt and Marine Chemicals Research Institute (CSIR-CSMCRI), Council of Scientific and Industrial Research (CSIR), G.B. Marg, Bhavnagar, Gujarat, 364002, India.
Mol Biol Rep. 2018 Dec;45(6):1745-1758. doi: 10.1007/s11033-018-4318-1. Epub 2018 Aug 29.
Cytochromes are important components of photosynthetic electron transport chain. Here we report on genetic transformation of Cytochrome c (UfCyt c) gene from Ulva fasciata Delile in tobacco for enhanced photosynthesis and growth. UfCyt c cDNA had an open reading frame of 330 bp encoding a polypeptide of 109 amino acids with a predicted molecular mass of 11.65 kDa and an isoelectric point of 5.21. UfCyt c gene along with a tobacco petE transit peptide sequence under control of CaMV35S promoter was transformed in tobacco through Agrobacterium mediated genetic transformation. Transgenic tobacco grew normal and exhibited enhanced growth as compared to wild type (WT) and vector control (VC) tobacco. Transgenic tobacco had higher contents of photosynthetic pigments and better ratios of photosynthetic pigments. The tobacco expressing UfCyt c gene exhibited higher photosynthetic rate and improved water use efficiency. Further activity of the water-splitting complex, photosystem II quantum yield, photochemical quenching, electron transfer rate, and photosynthetic yield were found comparatively higher in transgenic tobacco as compared to WT and VC tobacco. Alternatively basal quantum yield of non-photochemical processes in PSII and non-photochemical quenching were estimated lower in tobacco expressing UfCyt c gene. As a result of improved photosynthetic performance the transgenic tobacco had higher contents of sugar and starch, and exhibited comparatively better growth. To the best of our knowledge this is the first report on expression of UfCyt c gene from U. fasciata for improved photosynthesis and growth in tobacco.
细胞色素是光合电子传递链的重要组成部分。在此，我们报道了将来自孔石莼的细胞色素c（UfCyt c）基因导入烟草进行遗传转化，以增强光合作用和促进生长。UfCyt c cDNA具有330 bp的开放阅读框，编码一个由109个氨基酸组成的多肽，预测分子量为11.65 kDa，等电点为5.21。在CaMV35S启动子的控制下，UfCyt c基因与烟草petE转运肽序列一起通过农杆菌介导的遗传转化导入烟草。与野生型（WT）和载体对照（VC）烟草相比，转基因烟草生长正常且生长增强。转基因烟草具有更高的光合色素含量和更好的光合色素比例。表达UfCyt c基因的烟草表现出更高的光合速率和提高的水分利用效率。与WT和VC烟草相比，转基因烟草中水分裂解复合物的活性、光系统II量子产率、光化学猝灭、电子传递速率和光合产量相对更高。另外，在表达UfCyt c基因的烟草中，PSII中非光化学过程的基础量子产率和非光化学猝灭估计较低。由于光合性能的改善，转基因烟草具有更高的糖和淀粉含量，并表现出相对更好的生长。据我们所知，这是关于孔石莼UfCyt c基因在烟草中表达以改善光合作用和生长的首次报道。
