探测藻类细胞群体中通道视紫红质的电活性。
Probing Channelrhodopsin Electrical Activity in Algal Cell Populations.
机构信息
McGovern Medical School, The University of Texas Health Science Center at Houston, Houston, TX, USA.
出版信息
Methods Mol Biol. 2021;2191:85-96. doi: 10.1007/978-1-0716-0830-2_6.
Abstract
Photoelectric recording from populations of phototactic flagellate algae provides a means to study channelrhodopsin functions in vivo. Technical simplicity, versatility, high sensitivity, and reproducibility are the advantages of this assay over recording from individual algal cells by the suction pipette technique. Here we describe the principles and procedures of this assay.
摘要
光电记录趋光性鞭毛藻种群提供了一种在体内研究通道视紫红质功能的方法。与用吸管技术记录单个藻类细胞相比，该测定法具有技术简单、多功能、高灵敏度和可重复性等优点。本文介绍了该测定法的原理和步骤。
相似文献
1
Probing Channelrhodopsin Electrical Activity in Algal Cell Populations.探测藻类细胞群体中通道视紫红质的电活性。
Methods Mol Biol. 2021;2191:85-96. doi: 10.1007/978-1-0716-0830-2_6.
2
Photosensory functions of channelrhodopsins in native algal cells.天然藻类细胞中视紫红质通道蛋白的光感受功能。
Photochem Photobiol. 2009 Mar-Apr;85(2):556-63. doi: 10.1111/j.1751-1097.2008.00524.x. Epub 2009 Feb 11.
3
Channelrhodopsin-1 Phosphorylation Changes with Phototactic Behavior and Responds to Physiological Stimuli in .通道视紫红质-1 的磷酸化随趋光行为而变化，并对 作出生理响应。
Plant Cell. 2019 Apr;31(4):886-910. doi: 10.1105/tpc.18.00936. Epub 2019 Mar 12.
4
New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride.从绿藻中提取的具有红移光谱和快速动力学的新型通道视紫红质。
mBio. 2011 Jun 21;2(3):e00115-11. doi: 10.1128/mBio.00115-11. Print 2011.
5
Time-resolved serial femtosecond crystallography reveals early structural changes in channelrhodopsin.时间分辨连续飞秒晶体学揭示通道蛋白视紫红质的早期结构变化。
Elife. 2021 Mar 23;10:e62389. doi: 10.7554/eLife.62389.
6
Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis.从海洋藻类原甲藻中鉴定出一种高效的蓝光调控通道蛋白。
J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30.
7
Nanodisc Reconstitution of Channelrhodopsins Heterologously Expressed in Pichia pastoris for Biophysical Investigations.在毕赤酵母中异源表达的通道蛋白视紫红质的纳米盘重构用于生物物理研究。
Methods Mol Biol. 2021;2191:29-48. doi: 10.1007/978-1-0716-0830-2_3.
8
The form and function of channelrhodopsin.视紫红质通道蛋白的形式与功能。
Science. 2017 Sep 15;357(6356). doi: 10.1126/science.aan5544.
9
Channelrhodopsins provide a breakthrough insight into strategies for curing blindness.视紫红质通道蛋白为治愈失明的策略提供了突破性的见解。
J Genet. 2009 Dec;88(4):409-15. doi: 10.1007/s12041-009-0062-6.
10
Glu 87 of channelrhodopsin-1 causes pH-dependent color tuning and fast photocurrent inactivation.通道视紫红质-1的第87位谷氨酸导致pH依赖性颜色调谐和快速光电流失活。
Photochem Photobiol. 2009 Mar-Apr;85(2):564-9. doi: 10.1111/j.1751-1097.2008.00519.x. Epub 2009 Jan 19.
本文引用的文献
1
Targeting of Photoreceptor Genes in via Zinc-Finger Nucleases and CRISPR/Cas9.利用锌指核酸酶和 CRISPR/Cas9 靶向 。
Plant Cell. 2017 Oct;29(10):2498-2518. doi: 10.1105/tpc.17.00659. Epub 2017 Oct 4.
2
The form and function of channelrhodopsin.视紫红质通道蛋白的形式与功能。
Science. 2017 Sep 15;357(6356). doi: 10.1126/science.aan5544.
3
His166 is the Schiff base proton acceptor in attractant phototaxis receptor sensory rhodopsin I.组氨酸166是吸引性趋光受体感官视紫红质I中的席夫碱质子受体。
Biochemistry. 2014 Sep 23;53(37):5923-9. doi: 10.1021/bi500831n. Epub 2014 Sep 8.
4
Channelrhodopsin unchained: structure and mechanism of a light-gated cation channel.解锁的通道视紫红质：一种光门控阳离子通道的结构与机制
Biochim Biophys Acta. 2014 May;1837(5):626-42. doi: 10.1016/j.bbabio.2013.10.014. Epub 2013 Nov 7.
5
Functional analysis of the eyespot in Chlamydomonas reinhardtii mutant ey 627, mt (-).眼点在眼虫 Chlamydomonas reinhardtii 突变体 ey627,mt(-)中的功能分析。
Planta. 1992 Nov;188(4):513-21. doi: 10.1007/BF00197043.
6
Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes.寡聚体视紫质复合物中跨原体与光活性位点的相互作用。
Acta Crystallogr D Biol Crystallogr. 2013 Oct;69(Pt 10):1965-80. doi: 10.1107/S0907444913017575. Epub 2013 Sep 20.
7
Characterization of a highly efficient blue-shifted channelrhodopsin from the marine alga Platymonas subcordiformis.从海洋藻类原甲藻中鉴定出一种高效的蓝光调控通道蛋白。
J Biol Chem. 2013 Oct 11;288(41):29911-22. doi: 10.1074/jbc.M113.505495. Epub 2013 Aug 30.
8
New channelrhodopsin with a red-shifted spectrum and rapid kinetics from Mesostigma viride.从绿藻中提取的具有红移光谱和快速动力学的新型通道视紫红质。
mBio. 2011 Jun 21;2(3):e00115-11. doi: 10.1128/mBio.00115-11. Print 2011.
9
Optogenetics.光遗传学。
Nat Methods. 2011 Jan;8(1):26-9. doi: 10.1038/nmeth.f.324. Epub 2010 Dec 20.
10
Attractant and repellent signaling conformers of sensory rhodopsin-transducer complexes.感觉视紫红质转导复合物的吸引和排斥信号构象体。
Biochemistry. 2010 Aug 10;49(31):6696-704. doi: 10.1021/bi100798w.
Zotero 插件