光遗传学控制转基因小鼠心脏自主神经元。
Optogenetic Control of Cardiac Autonomic Neurons in Transgenic Mice.
机构信息
Department of Biomedical Engineering, The George Washington University, Washington, DC, USA.
Department of Pharmacology and Physiology, The George Washington University, Washington, DC, USA.
出版信息
Methods Mol Biol. 2021;2191:309-321. doi: 10.1007/978-1-0716-0830-2_18.
Optogenetic technology has enabled unparalleled insights into cellular and organ physiology by providing exquisite temporal and spatial control of biological pathways. Here, an optogenetic approach is presented for selective activation of the intrinsic cardiac nervous system in excised perfused mouse hearts. The breeding of transgenic mice that have selective expression of channelrhodopsin in either catecholaminergic or cholinergic neurons is described. An approach for perfusing hearts excised from those animals, recording the ECG to measure heart rate changes, and an illumination technique using a custom micro-LED light source to activate channelrhodopsin is explained. We have used these methods in ongoing studies of the kinetics of autonomic control of cardiac electrophysiology and contractility, demonstrating the proven utility of optogenetic technology to enable unparalleled spatiotemporal anatomic-functional probing of the intrinsic cardiac nervous system.
光遗传学技术通过对生物途径进行精确的时空控制，为研究细胞和器官生理学提供了无与伦比的见解。在这里，我们提出了一种光遗传学方法，用于选择性激活离体灌流小鼠心脏中的固有心脏神经系统。描述了一种在儿茶酚胺能神经元或胆碱能神经元中选择性表达通道视紫红质的转基因小鼠的繁殖方法。还介绍了从这些动物中取出心脏进行灌流、记录心电图以测量心率变化的方法，以及使用定制微 LED 光源进行光照的技术，以激活通道视紫红质。我们已经在正在进行的自主控制心脏电生理学和收缩性的动力学研究中使用了这些方法，证明了光遗传学技术在对固有心脏神经系统进行前所未有的时空解剖功能探测方面的实用性。
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