Institute of Basic Medical Science, Hubei University of Medicine, PR China; Hubei Key Laboratory of Embryonic Stem Cell Research, Hubei University of Medicine, PR China.
Department of General Practice, Renmin Hospital, Hubei University of Medicine, PR China.
Virus Res. 2020 Nov;289:198149. doi: 10.1016/j.virusres.2020.198149. Epub 2020 Aug 29.
The pathogenesis of Enterovirus 71 (EV71)-induced severe hand foot and mouth disease (HFMD) has not yet been clearly established. Further study into the mechanisms underlying host immune responses to EV71 infection and identifying important predictors will be crucial to antiviral treatment and early recognition of severe HFMD. The present study establishes that T help (Th)1 type, Th2 type, and Th17 type cytokine levels in serum of peripheral blood from patients with severe HFMD is higher than in peripheral blood from healthy subjects. The most significant increase occurred as the IL-6. In order to identify the important molecules in peripheral blood mononuclear cells (PBMCs) from severe HFMD patients, we performed transcriptome sequencing analysis of PBMC from severe HFMD patients and compared them to healthy controls. Interferon α-inducible protein 27 (IFI27) and cluster of differentiation 27 (CD27) were found to be the most significant differentially expressed gene. Finally, IFI27 was proved to be present at higher levels in patients with severe HFMD than in patients with mild HFMD. Our results suggest that IFI27 may be an indicator of the severity of cases EV71-induced HFMD.
肠道病毒 71 型（EV71）引起的重症手足口病（HFMD）的发病机制尚未明确。进一步研究宿主对 EV71 感染的免疫反应机制，并确定重要的预测因子，对于抗病毒治疗和早期识别重症 HFMD 至关重要。本研究表明，重症 HFMD 患者外周血血清中的辅助性 T 细胞（Th）1 型、Th2 型和 Th17 型细胞因子水平高于健康受试者。其中 IL-6 升高最为显著。为了鉴定重症 HFMD 患者外周血单个核细胞（PBMC）中的重要分子，我们对重症 HFMD 患者的 PBMC 进行了转录组测序分析，并与健康对照进行了比较。发现干扰素诱导蛋白 27（IFI27）和分化抗原 27（CD27）是差异表达最显著的基因。最后，证明 IFI27 在重症 HFMD 患者中的表达水平高于轻症 HFMD 患者。我们的结果表明，IFI27 可能是 EV71 引起的 HFMD 严重程度的指标。
