State Key Laboratory of Silkworm Genome Biology, College of Sericulture, Textile and Biomass Sciences, Southwest University, Chongqing 400715, China.
Int J Mol Sci. 2020 Aug 27;21(17):6180. doi: 10.3390/ijms21176180.
Diapause is a form of dormancy, and silkworm embryos are ideal models for studying diapause in insects. However, molecular events in eggs during the onset of diapause remain unclear. In this study, transcriptome analyses were performed on silkworm diapause eggs via RNA sequencing at 20 and 48 h after oviposition. A total of 6402 differentially expressed genes (DEGs) were detected in diapause eggs at 48 h versus that at 20 h after oviposition. Gene ontology enrichment analysis showed that DEGs in diapause eggs at 48 h versus that at 20 h after oviposition were involved in ribosome-related metabolism and hydrogen transport. Kyoto Encyclopedia of Genes and Genomes analysis revealed several significantly enriched biological pathways, namely the oxidative phosphorylation, Forkhead box protein O3 (FoxO) signaling, ribosome, endoplasmic reticular protein processing, and autophagy pathways. Fifteen DEGs from the FoxO signaling pathway were selected, and their expression profiles were consistent with the transcriptome results from real-time quantitative reverse transcription polymerase chain reaction. Our results can improve understanding of the diapause mechanism in silkworm eggs and identified key pathways for future studies.
滞育是一种休眠形式，而家蚕胚胎是研究昆虫滞育的理想模型。然而，卵在滞育开始时的分子事件仍不清楚。本研究通过 RNA 测序在产卵后 20 和 48 小时对家蚕滞育卵进行了转录组分析。在产卵后 48 小时的滞育卵中，共检测到 6402 个差异表达基因（DEGs）。基因本体富集分析表明，在产卵后 48 小时的滞育卵中，DEGs 参与核糖体相关代谢和氢运输。京都基因与基因组百科全书分析揭示了几个显著富集的生物途径，即氧化磷酸化、叉头框蛋白 O3（FoxO）信号、核糖体、内质网蛋白加工和自噬途径。从 FoxO 信号通路中选择了 15 个 DEGs，它们的表达谱与实时定量逆转录聚合酶链反应的转录组结果一致。我们的研究结果可以提高对家蚕卵滞育机制的理解，并为未来的研究确定关键途径。
