Adegbaju Muyiwa S, Morenikeji Olanrewaju B, Borrego Eli J, Hudson André O, Thomas Bolaji N
Institute for Plant Biotechnology, Stellenbosch University, Stellenbosch 7600, South Africa.
Department of Biomedical Sciences, College of Health Science and Technology, Rochester Institute of Technology, Rochester, NY 14623, USA.
Plants (Basel). 2020 Aug 27;9(9):1101. doi: 10.3390/plants9091101.
The alpha-glucan water dikinase (GWD) enzyme catalyzes starch phosphorylation, an integral step in transitory starch degradation. The high phosphate content in stored starch has great industrial value, due to its physio-chemical properties making it more versatile, although the phosphate content of stored starch varies depending on the botanical source. In this study, we used various computational approaches to gain insights into the evolution of the GWD protein in 48 plant species with possible roles in enzyme function and alteration of phosphate content in their stored starch. Our analyses identified deleterious mutations, particularly in the highly conserved 5 aromatic amino acid residues in the dual tandem carbohydrate binding modules (CBM-45) of GWD protein in , , , , and These findings will inform experimental designs for simultaneous repression of genes coding for GWD and the predicted interacting proteins to elucidate the role this enzyme plays in starch degradation. Our results reveal significant diversity in the evolution of GWD enzyme across plant species, which may be evolutionarily advantageous according to the varying needs for phosphorylated stored starch between plants and environments.
α-葡聚糖水二激酶（GWD）催化淀粉磷酸化，这是瞬时淀粉降解过程中的一个重要步骤。储存淀粉中的高磷酸盐含量具有很大的工业价值，因为其物理化学性质使其用途更广泛，尽管储存淀粉的磷酸盐含量因植物来源而异。在本研究中，我们使用了各种计算方法来深入了解48种植物中GWD蛋白的进化情况，这些植物可能在酶功能以及其储存淀粉中磷酸盐含量的改变方面发挥作用。我们的分析确定了有害突变，特别是在GWD蛋白双串联碳水化合物结合模块（CBM-45）中高度保守的5个芳香族氨基酸残基中，在[此处可能遗漏了一些植物名称]中。这些发现将为同时抑制编码GWD和预测的相互作用蛋白的基因的实验设计提供信息，以阐明该酶在淀粉降解中所起的作用。我们的结果揭示了GWD酶在不同植物物种进化过程中的显著多样性，根据植物和环境对磷酸化储存淀粉的不同需求，这可能在进化上具有优势。
