• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

ROS 的局部积累可以阻断效应因子 BAS4 的表达，从而防止水稻感染。

Focal Accumulation of ROS Can Block Effector BAS4-Expression and Prevent Infection in Rice.

机构信息

Division of Integrative Bioscience and Biotechnology, College of Life Sciences, Sejong University, Seoul 05006, Korea.

Department of Plant Physiology, Swammerdam Institute for Life Sciences, University of Amsterdam, 1090 GE Amsterdam, The Netherlands.

出版信息

Int J Mol Sci. 2020 Aug 27;21(17):6196. doi: 10.3390/ijms21176196.

DOI:10.3390/ijms21176196
PMID:32867341
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7503722/
Abstract

The reactive oxygen species (ROS) burst is the most common plant immunity mechanism to prevent pathogen infection, although the exact role of ROS in plant immunity has not been fully elucidated. We investigated the expression and translocation of respiratory burst oxidase homologue B (OsRBOHB) during compatible and incompatible interactions between rice epidermal cells and the pathogenic fungus (syn. ). We characterized the functional role of ROS focal accumulation around invading hyphae during infection process using the OsRBOHB inhibitor diphenyleneiodonium (DPI) and the actin filament polymerization inhibitor cytochalasin (Cyt) A. OsRBOHB was strongly induced during incompatible rice- interactions, and newly synthesized OsRBOHB was focally distributed at infection sites. High concentrations of ROS focally accumulated at the infection sites and suppressed effector biotrophy-associated secreted (BAS) proteins BAS4 expression and invasive hyphal growth. DPI and Cyt A abolished ROS focal accumulation and restored effector BAS4 expression. These results suggest that ROS focal accumulation is able to function as an effective immune mechanism that blocks some effectors including BAS4-expression during infection. Disruption of ROS focal accumulation around invading hyphae enables successful colonization of rice cells and disease development.

摘要

活性氧（ROS）爆发是植物防止病原体感染的最常见免疫机制，尽管 ROS 在植物免疫中的确切作用尚未完全阐明。我们研究了呼吸爆发氧化酶同源物 B（OsRBOHB）在水稻表皮细胞与致病性真菌（syn.）之间的亲和和非亲和相互作用过程中的表达和易位。我们使用 ROS 爆发氧化酶同源物 B（OsRBOHB）抑制剂二苯基碘（DPI）和肌动蛋白丝聚合抑制剂细胞松弛素（Cyt）A 研究了 ROS 焦点积累在感染过程中对侵入菌丝的功能作用。在亲和性水稻相互作用中，OsRBOHB 被强烈诱导，新合成的 OsRBOHB 集中分布在感染部位。ROS 焦点在感染部位高度聚集，并抑制效应生物营养相关分泌（BAS）蛋白 BAS4 的表达和侵入菌丝的生长。DPI 和 Cyt A 消除了 ROS 焦点的积累并恢复了效应 BAS4 的表达。这些结果表明，ROS 焦点的积累能够作为一种有效的免疫机制，在感染过程中阻止包括 BAS4 表达在内的一些效应子。破坏侵入菌丝周围 ROS 焦点的积累使成功定殖水稻细胞和疾病发展成为可能。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/7a83ea0c26b2/ijms-21-06196-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/f29ff291f685/ijms-21-06196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/9da117c90c67/ijms-21-06196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/80e4f3f03789/ijms-21-06196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/e80f1933c757/ijms-21-06196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/f3fc5a145307/ijms-21-06196-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/7a83ea0c26b2/ijms-21-06196-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/f29ff291f685/ijms-21-06196-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/9da117c90c67/ijms-21-06196-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/80e4f3f03789/ijms-21-06196-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/e80f1933c757/ijms-21-06196-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/f3fc5a145307/ijms-21-06196-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6197/7503722/7a83ea0c26b2/ijms-21-06196-g006.jpg

相似文献

1
Focal Accumulation of ROS Can Block Effector BAS4-Expression and Prevent Infection in Rice.ROS 的局部积累可以阻断效应因子 BAS4 的表达，从而防止水稻感染。
Int J Mol Sci. 2020 Aug 27;21(17):6196. doi: 10.3390/ijms21176196.
2
Every Coin Has Two Sides: Reactive Oxygen Species during Rice⁻ Interaction.每枚硬币都有两面：水稻互作过程中的活性氧。
Int J Mol Sci. 2019 Mar 8;20(5):1191. doi: 10.3390/ijms20051191.
3
OsATL32 ubiquitinates the reactive oxygen species-producing OsRac5-OsRbohB module to suppress rice immunity.OsATL32 泛素化活性氧产生的 OsRac5-OsRbohB 模块，以抑制水稻的免疫反应。
J Integr Plant Biol. 2024 Jul;66(7):1459-1480. doi: 10.1111/jipb.13666. Epub 2024 Apr 17.
4
The Magnaporthe oryzae effector AvrPiz-t targets the RING E3 ubiquitin ligase APIP6 to suppress pathogen-associated molecular pattern-triggered immunity in rice.稻瘟病菌效应蛋白 AvrPiz-t 靶向 RING E3 泛素连接酶 APIP6 抑制水稻的病原相关分子模式触发的免疫。
Plant Cell. 2012 Nov;24(11):4748-62. doi: 10.1105/tpc.112.105429. Epub 2012 Nov 30.
5
Magnaporthe oryzae Effector AVR-Pii Helps to Establish Compatibility by Inhibition of the Rice NADP-Malic Enzyme Resulting in Disruption of Oxidative Burst and Host Innate Immunity.稻瘟病菌效应蛋白AVR - Pii通过抑制水稻NADP - 苹果酸酶来破坏氧化爆发和宿主先天免疫，从而有助于建立亲和性。
Mol Cells. 2016 May 31;39(5):426-38. doi: 10.14348/molcells.2016.0094. Epub 2016 May 3.
6
Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice- Interactions.铁和活性氧依赖的水稻细胞铁死亡相互作用。
Plant Cell. 2019 Jan;31(1):189-209. doi: 10.1105/tpc.18.00535. Epub 2018 Dec 18.
7
A pharmacological approach to investigating effector translocation in rice- oryzae interactions.一种研究水稻-稻瘟病菌相互作用中效应子易位的药理学方法。
Plant Signal Behav. 2024 Dec 31;19(1):2350869. doi: 10.1080/15592324.2024.2350869. Epub 2024 May 9.
8
Interaction transcriptome analysis identifies Magnaporthe oryzae BAS1-4 as Biotrophy-associated secreted proteins in rice blast disease.相互作用转录组分析确定稻瘟病菌BAS1-4为水稻稻瘟病中与活体营养相关的分泌蛋白。
Plant Cell. 2009 Apr;21(4):1273-90. doi: 10.1105/tpc.107.055228. Epub 2009 Apr 7.
9
The biotrophy-associated secreted protein 4 (BAS4) participates in the transition of from the biotrophic to the necrotrophic phase.生物营养相关分泌蛋白4（BAS4）参与从生物营养阶段到坏死营养阶段的转变。
Saudi J Biol Sci. 2019 May;26(4):795-807. doi: 10.1016/j.sjbs.2019.01.003. Epub 2019 Jan 8.
10
Overexpression of Systemic Defense Trigger 1 (MoSDT1) Confers Improved Rice Blast Resistance in Rice.过量表达系统防御触发 1（MoSDT1）赋予水稻增强的稻瘟病抗性。
Int J Mol Sci. 2019 Sep 25;20(19):4762. doi: 10.3390/ijms20194762.

引用本文的文献

1
Phenazine biosynthesis protein MoPhzF regulates appressorium formation and host infection through canonical metabolic and noncanonical signaling function in Magnaporthe oryzae.稻瘟病菌苯并嗪生物合成蛋白 MoPhzF 通过经典代谢和非经典信号功能调控附着胞形成和侵染宿主。
New Phytol. 2024 Apr;242(1):211-230. doi: 10.1111/nph.19569. Epub 2024 Feb 7.
2
Phosphate accumulation in rice leaves promotes fungal pathogenicity and represses host immune responses during pathogen infection.水稻叶片中的磷酸盐积累在病原体感染期间促进真菌致病性并抑制宿主免疫反应。
Front Plant Sci. 2024 Jan 17;14:1330349. doi: 10.3389/fpls.2023.1330349. eCollection 2023.
3

本文引用的文献

1
Every Coin Has Two Sides: Reactive Oxygen Species during Rice⁻ Interaction.每枚硬币都有两面：水稻互作过程中的活性氧。
Int J Mol Sci. 2019 Mar 8;20(5):1191. doi: 10.3390/ijms20051191.
2
Ferroptosis: A Companion of ROS in Fighting in Rice.铁死亡：水稻中活性氧对抗过程中的一个“伙伴”
Plant Cell. 2019 Jan;31(1):13-14. doi: 10.1105/tpc.18.00970. Epub 2019 Jan 3.
3
Iron- and Reactive Oxygen Species-Dependent Ferroptotic Cell Death in Rice- Interactions.铁和活性氧依赖的水稻细胞铁死亡相互作用。
Recent Advances in Effector Research of .
近期. 的效应子研究进展。
Biomolecules. 2023 Nov 14;13(11):1650. doi: 10.3390/biom13111650.
4
The Cytoskeleton in Plant Immunity: Dynamics, Regulation, and Function.植物免疫中的细胞骨架：动态、调节与功能。
Int J Mol Sci. 2022 Dec 8;23(24):15553. doi: 10.3390/ijms232415553.
5
β-Carboline Alkaloids from Inhibit from through Damaging the Cell Membrane and Inducing ROS Accumulation.来自[具体来源未明确]的β-咔啉生物碱通过破坏细胞膜和诱导活性氧积累来抑制[具体抑制对象未明确]。
Pathogens. 2022 Nov 13;11(11):1341. doi: 10.3390/pathogens11111341.
6
The NIN-Like Protein OsNLP2 Negatively Regulates Ferroptotic Cell Death and Immune Responses to in Rice.类核仁蛋白OsNLP2对水稻中铁死亡细胞死亡和免疫反应起负调控作用。
Antioxidants (Basel). 2022 Sep 12;11(9):1795. doi: 10.3390/antiox11091795.
7
Integrated transcriptomic and transgenic analyses reveal potential mechanisms of poplar resistance to Alternaria alternata infection.综合转录组学和转基因分析揭示了杨树对链格孢菌感染抗性的潜在机制。
BMC Plant Biol. 2022 Aug 25;22(1):413. doi: 10.1186/s12870-022-03793-5.
8
Co-evolved plant and blast fungus ascorbate oxidases orchestrate the redox state of host apoplast to modulate rice immunity.协同进化的植物和病原菌抗坏血酸氧化酶调控宿主质外体的氧化还原状态从而调节水稻免疫。
Mol Plant. 2022 Aug 1;15(8):1347-1366. doi: 10.1016/j.molp.2022.07.001. Epub 2022 Jul 6.
Plant Cell. 2019 Jan;31(1):189-209. doi: 10.1105/tpc.18.00535. Epub 2018 Dec 18.
4
Quantitative phosphoproteomic analysis reveals common regulatory mechanisms between effector- and PAMP-triggered immunity in plants.定量磷酸化蛋白质组学分析揭示了植物效应器和病原体相关分子模式触发免疫之间的共同调控机制。
New Phytol. 2019 Mar;221(4):2160-2175. doi: 10.1111/nph.15523. Epub 2018 Nov 5.
5
A nuclear contortionist: the mitotic migration of nuclei during plant infection.一位核“柔术演员”：植物感染期间细胞核的有丝分裂迁移
Mycology. 2018 Jun 12;9(3):202-210. doi: 10.1080/21501203.2018.1482966. eCollection 2018.
6
Tools of the crook- infection strategies of fungal plant pathogens.骗子的工具——真菌植物病原体的侵染策略。
Plant J. 2018 Feb;93(4):664-674. doi: 10.1111/tpj.13810. Epub 2018 Jan 29.
7
Convergent Evolution of Pathogen Effectors toward Reactive Oxygen Species Signaling Networks in Plants.病原体效应蛋白向植物活性氧信号网络的趋同进化
Front Plant Sci. 2017 Sep 29;8:1687. doi: 10.3389/fpls.2017.01687. eCollection 2017.
8
An NADPH Oxidase RBOH Functions in Rice Roots during Lysigenous Aerenchyma Formation under Oxygen-Deficient Conditions.一种NADPH氧化酶RBOH在缺氧条件下水稻根部溶生性通气组织形成过程中发挥作用。
Plant Cell. 2017 Apr;29(4):775-790. doi: 10.1105/tpc.16.00976. Epub 2017 Mar 28.
9
Homologues of the RPW8 Resistance Protein Are Localized to the Extrahaustorial Membrane that Is Likely Synthesized De Novo.RPW8抗性蛋白的同源物定位于可能重新合成的吸器外膜。
Plant Physiol. 2017 Jan;173(1):600-613. doi: 10.1104/pp.16.01539. Epub 2016 Nov 17.
10
Investigating the cell biology of plant infection by the rice blast fungus Magnaporthe oryzae.研究稻瘟病菌对水稻感染的细胞生物学。
Curr Opin Microbiol. 2016 Dec;34:147-153. doi: 10.1016/j.mib.2016.10.001. Epub 2016 Nov 3.