The Department of Plastic and Burn Surgery, Shenzhen Hospital, Southern Medical University , Shenzhen, China.
The Third School of Clinical Medicine, Southern Medical University , Shenzhen, China.
Biosci Biotechnol Biochem. 2020 Dec;84(12):2521-2528. doi: 10.1080/09168451.2020.1807901. Epub 2020 Aug 31.
Fifteen percent third-degree burn rat model was used to identify miRNAs that are markers of burn injury-induced myocardial damage. Cardiac tissues were evaluated to determine miRNA profile sequencing. Pearson's correlation analysis was used between miRNAs and injury markers. ROC curve analysis was used to estimate miRNA's sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. The sequencing analysis revealed 23 differentially expressed miRNAs. Pearson's correlation analysis revealed that rno-miR-190b-3p and C5b9, rno-miR-341, rno-miR-344b-3p and TnI, rno-miR-344b-3p and CK-MB were significantly positively correlated, respectively. ROC curve analysis demonstrated that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p exhibited high sensitivity and specificity for the diagnosis of myocardial damage caused by burn injury. In conclusion, our results suggest that rno-miR-341, rno-miR-344b-3p, and rno-miR-190b-3p have the potential to be used as sensitive and specific biomarkers to diagnose myocardial damage caused by burn injury.
采用 15%的三度烧伤大鼠模型来鉴定可作为烧伤诱导心肌损伤标志物的 miRNA。评估心脏组织以确定 miRNA 谱测序。使用 Pearson 相关分析在 miRNA 和损伤标志物之间进行相关性分析。使用 ROC 曲线分析来评估 miRNA 对烧伤引起的心肌损伤的诊断的敏感性和特异性。测序分析显示 23 个差异表达的 miRNA。Pearson 相关分析显示，rno-miR-190b-3p 和 C5b9、rno-miR-341、rno-miR-344b-3p 和 TnI、rno-miR-344b-3p 和 CK-MB 分别呈显著正相关。ROC 曲线分析表明，rno-miR-341、rno-miR-344b-3p 和 rno-miR-190b-3p 对烧伤引起的心肌损伤的诊断具有较高的敏感性和特异性。总之，我们的结果表明，rno-miR-341、rno-miR-344b-3p 和 rno-miR-190b-3p 具有作为烧伤引起的心肌损伤的敏感和特异性生物标志物的潜力。
