Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong 271018, China.
College of Animal Science and Technology, Shandong Agricultural University, Tai'an, Shandong 271018, China; Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong 271018, China; Shandong Provincial Engineering Technology Research Center of Animal Disease Control and Prevention, Shandong Agricultural University, Tai'an, Shandong 271018, China.
Poult Sci. 2020 Sep;99(9):4323-4333. doi: 10.1016/j.psj.2020.05.041. Epub 2020 Jun 26.
Since 2017, a serious infectious disease characterized by visceral gout has emerged in China's main goose-producing regions. The disease has caused huge economic losses to China's goose industry. In our previous study, we determined that the pathogen causing gout in goslings is a novel goose-origin astrovirus, designated as AStV/SDPY/Goose/1116/17 (AStV-SDPY) strain. To investigate the effect of host age on the outcome of novel goose-origin astrovirus infection, 200 1-day-old healthy goslings were selected to be experimentally infected at 1, 5, 15, 25, and 35 D of age. It was shown in experimental infection that the AStV-SDPY strain was highly pathogenic in goslings aged 1 to 15 D, causing growth repression, severe visceral urate deposition, and even death, whereas goslings infected at 25 and 35 D of age showed mild symptoms. Histopathologic examination indicated that lesions occurred mainly in the kidney and liver of infected goslings, which is correlated to the severity of clinical signs and gross lesions. Viral RNA was detected in all representative tissues, and virus shedding was detected continuously within 15 D after inoculation. Higher viral copy number, especially in vital organs such as the liver and kidney, was developed in the goslings infected at 1 to 15 D of age than older geese. In addition, clinical chemistry and inflammatory cytokines showed that younger geese are more sensitive to AStV infection. Overall, our study demonstrates that the pathogenicity of AStV-SDPY in goslings is partly associated with the age of infection, laying a foundation for further study of the pathogenic mechanism of this virus.
自 2017 年以来，中国主要鹅产区出现了一种以内脏痛风为特征的严重传染病。该疾病给中国鹅业造成了巨大的经济损失。在我们之前的研究中，我们确定引起雏鹅痛风的病原体是一种新型鹅源星状病毒，命名为 AStV/SDPY/Goose/1116/17（AStV-SDPY）株。为了研究宿主年龄对新型鹅源星状病毒感染结果的影响，选择 200 只 1 日龄健康雏鹅，分别于 1、5、15、25 和 35 日龄进行人工感染。实验感染结果表明，AStV-SDPY 株在 1 至 15 日龄雏鹅中具有高致病性，导致生长抑制、严重内脏尿酸盐沉积，甚至死亡，而 25 和 35 日龄感染的雏鹅症状较轻。组织病理学检查表明，感染雏鹅的病变主要发生在肾脏和肝脏，这与临床症状和大体病变的严重程度有关。在所有代表性组织中均检测到病毒 RNA，接种后 15 天内连续检测到病毒脱落。1 至 15 日龄感染雏鹅的病毒拷贝数较高，特别是在肝脏和肾脏等重要器官，比年龄较大的鹅更为明显。此外，临床化学和炎症细胞因子表明，幼鹅对 AStV 感染更为敏感。总之，本研究表明 AStV-SDPY 在雏鹅中的致病性部分与感染年龄有关，为进一步研究该病毒的致病机制奠定了基础。
