College of Animal Science and Technology, Shandong Agricultural University, 61 Daizong Street, Tai'an, 271018, Shandong, China.
Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control and Prevention, Tai'an, 271018, Shandong, China.
BMC Vet Res. 2021 Jan 20;17(1):40. doi: 10.1186/s12917-020-02739-z.
A novel goose-origin astrovirus (GoAstV) has broken out across China in recent years, causing gout in goslings with a mortality rate of around 50%. However, our understanding of the dynamic distribution, tissue tropism and pathogenesis of GoAstV is incomplete. In order to assess its pathogenicity, one-day-old goslings were inoculated separately with GoAstV via oral and subcutaneous injection routes.
Clinical symptoms, gross and microscopic lesions, blood biochemical parameters and viral loads were detected and recorded for 20 days after infection. Typical gout was observed in experimental goslings. GoAstV can be replicated in tissues and cause pathological damage, especially in the kidney, liver, heart and spleen. Virus-specific genomic RNA was detected in blood, cloacal swabs and all representative tissues, and virus shedding was detected up to 20 days after inoculation, suggesting that GoAstV has a wide tissue tropism and spread systematically after inoculation. The viral copy numbers examined in kidney were the highest, followed by spleen and liver.
This experiment determined the accurate value of viral loads and biochemical indicators of GoAstV-induced goslings. These findings increase our understanding of the pathogenicity of GoAstV in goslings and provide more reference for future research.
近年来，一种新型的鹅源星状病毒（GoAstV）在中国各地爆发，导致鹅痛风，死亡率约为 50%。然而，我们对 GoAstV 的动态分布、组织嗜性和发病机制的了解还不完整。为了评估其致病性，我们通过口服和皮下注射途径分别将 GoAstV 接种给 1 日龄雏鹅。
感染后 20 天内，检测并记录了临床症状、大体和显微镜病变、血液生化参数和病毒载量。实验鹅出现典型痛风。GoAstV 可在组织中复制并引起病理损伤，尤其是肾脏、肝脏、心脏和脾脏。在血液、泄殖腔拭子和所有代表性组织中均检测到病毒特异性基因组 RNA，并且在接种后 20 天内检测到病毒脱落，表明 GoAstV 具有广泛的组织嗜性，并在接种后系统性传播。检测到的肾脏中的病毒拷贝数最高，其次是脾脏和肝脏。
本实验确定了 GoAstV 诱导雏鹅的病毒载量和生化指标的准确值。这些发现增加了我们对 GoAstV 在雏鹅中的致病性的理解，并为未来的研究提供了更多参考。
