Zheng Ya-Wen, Wu Ming-Yue, Shen Xue-Yong, Wang Li-Na
School of Acupuncture-moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China.
School of Acupuncture-moxibustion and Tuina, Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China; Shanghai Research Center for Acupuncture and Meridians, Shanghai 201203.
Zhen Ci Yan Jiu. 2020 Aug 25;45(8):645-51. doi: 10.13702/j.1000-0607.190703.
To compare the analgesic effect of manual acupuncture(MA) stimulation of "Zusanli" (ST36) in rats with inflammatory pain under unrestrained conscious, restrained and general anesthesia conditions, so as to explore the applicability of unrestrained conscious model in the evaluation of acupuncture analgesia effect.
Male SD rats were divided into 5 groups: blank control (=9), pain model (=7), unrestrained conscious conditions+MA (=6), restrained conditions+MA (=6), and general anesthesia (GA)+MA(=6). The acute pain model was established by injection of complete Freund's adjuvant (CFA) into the left ankle joint 48 h ahead of acupuncture. Subsequently, a single 20 min session of MA was applied to the left ST36. The mechanical and thermal pain thresholds (MPT and TPT) were determined before and after injection of CFA, and after MA stimulation. In order to evaluate the autonomic behavior activities, rats were randomly divided into blank control (=11), pain model (=11) and conscious-unrestrained conditions +MA (=12) groups. The rats' exploratory movements were assessed by open field tests.
Both MPT and TPT were significantly decreased after injection of CFA in the model group relevant to the blank control group (<0.001), and significantly higher in the three MA groups than in the model group (<0.001). Comparison among the three MA groups showed that both MPT and TPT were significantly higher in the conscious unrestrained conditions+MA group than in the restrained conditions+MA and GA+MA groups (<0.05, <0.01). Open filed tests showed that the total moving distance in the open field and wall climbing times were significantly lower in the model group than in the blank control group (<0.01), and the wall climbing times were obviously more in the unstrained conditions+MA group than in the model group (<0.05). The central area resistance time was significantly shorter in the model group than in the control group (<0.05), and was moderately increased after MA despite no evident significance (>0.05). No significant changes were found in the total moving distance after MA and in the central area moving distance after modeling and MA (>0.05).
MA has a better therapeutic effect in relieving pain and pain-induced depression-like behavior in conscious unrestrained rats than in restrained and GA rats, implying a higher applicability of unrestrained conscious pain model to the assessment of acupuncture analgesia.
比较手针刺激大鼠“足三里”（ST36）在自由清醒、束缚及全身麻醉状态下对炎性疼痛的镇痛效果，以探讨自由清醒模型在针刺镇痛效果评价中的适用性。
将雄性SD大鼠分为5组：空白对照组（n = 9）、疼痛模型组（n = 7）、自由清醒状态+手针组（n = 6）、束缚状态+手针组（n = 6）和全身麻醉（GA）+手针组（n = 6）。在针刺前48 h，通过向左侧踝关节注射完全弗氏佐剂（CFA）建立急性疼痛模型。随后，对左侧ST36进行单次20分钟的手针刺激。在注射CFA前、后以及手针刺激后测定机械性和热痛阈值（MPT和TPT）。为评估自主行为活动，将大鼠随机分为空白对照组（n = 11）、疼痛模型组（n = 11）和自由清醒状态+手针组（n = 12）。通过旷场试验评估大鼠的探索性运动。
与空白对照组相比，模型组注射CFA后MPT和TPT均显著降低（<0.001），三个手针组的MPT和TPT均显著高于模型组（<0.001）。三个手针组之间比较显示，自由清醒状态+手针组的MPT和TPT均显著高于束缚状态+手针组和GA+手针组（<0.05，<0.01）。旷场试验显示，模型组的旷场总移动距离和攀爬次数均显著低于空白对照组（<0.01），自由清醒状态+手针组的攀爬次数明显多于模型组（<0.05）。模型组的中央区域停留时间显著短于对照组（<0.05），手针刺激后虽无明显统计学意义但有适度增加（>0.05）。手针刺激后总移动距离以及造模和手针刺激后中央区域移动距离均无显著变化（>0.05）。
与束缚和全身麻醉的大鼠相比，手针刺激对自由清醒大鼠的疼痛及疼痛诱导的抑郁样行为具有更好的治疗效果，这意味着自由清醒疼痛模型在针刺镇痛评估中具有更高的适用性。
