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卷柏提取物通过 STAT3 和 Akt/NF-κB 信号通路诱导喉癌细胞凋亡

A biflavonoid-rich extract from Selaginella moellendorffii Hieron. induces apoptosis via STAT3 and Akt/NF-κB signalling pathways in laryngeal carcinoma.

机构信息

School of Pharmaceutical Sciences, South-Central University for Nationalities, Wuhan, China.

Hotian Uygur Pharmaceutical Co., Ltd, Hotian, China.

出版信息

J Cell Mol Med. 2020 Oct;24(20):11922-11935. doi: 10.1111/jcmm.15812. Epub 2020 Sep 1.

DOI:10.1111/jcmm.15812
PMID:32869923
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7579697/
Abstract

Selaginella moellendorffii Hieron. (SM), a perennial evergreen plant, has been used in the treatment of acute infectious hepatitis, thoracic and hypochondriac lumbar contusions, systemic oedema and thrombocytopaenia. However, the role of a biflavonoid-rich extract from SM (SM-BFRE) in anti-larynx cancer has rarely been reported. In this study, the in vitro and in vivo anti-laryngeal cancer activity and potential mechanisms of SM-BFRE were investigated. An off-line semipreparative liquid chromatography-nuclear magnetic resonance protocol was carried out to determine six biflavonoids from SM-BFRE. In vitro, MTT assay revealed that SM-BFRE inhibited the proliferation of laryngeal carcinoma cells. A wound healing assay indicated that SM-BFRE suppressed the migration of laryngeal cancer cells. Hoechst 33 258 and Annexin V-FITC/PI double staining assays were performed and verified that SM-BFRE induced apoptosis in laryngeal carcinoma cells. The Hep-2 bearing nude mouse model confirmed that SM-BFRE also exhibited anticancer effect in vivo. In addition, Western blot analysis demonstrated that SM-BFRE exerted its anti-laryngeal cancer effect by activating the mitochondrial apoptotic pathway and inhibiting STAT3 and Akt/NF-κB signalling pathways. All results suggested that SM-BFRE could be considered as a potential chemotherapeutic drug for laryngeal cancer.

摘要

卷柏（Selaginella moellendorffii Hieron.），一种多年生常绿植物，已被用于治疗急性传染性肝炎、胸腰椎挫伤、全身性水肿和血小板减少症。然而，卷柏中富含双黄酮的提取物（SM-BFRE）在抗喉癌方面的作用却鲜有报道。在这项研究中，研究了 SM-BFRE 的体外和体内抗喉癌活性及其潜在机制。采用离线半制备液相色谱-核磁共振方案从 SM-BFRE 中鉴定出六种双黄酮。体外 MTT 测定表明 SM-BFRE 抑制喉癌细胞的增殖。划痕愈合实验表明 SM-BFRE 抑制喉癌细胞的迁移。Hoechst 33258 和 Annexin V-FITC/PI 双重染色实验证实 SM-BFRE 诱导喉癌细胞凋亡。在 Hep-2 荷瘤裸鼠模型中也证实了 SM-BFRE 在体内具有抗癌作用。此外，Western blot 分析表明，SM-BFRE 通过激活线粒体凋亡途径和抑制 STAT3 和 Akt/NF-κB 信号通路发挥其抗喉癌作用。所有结果表明，SM-BFRE 可被视为一种有潜力的喉癌化疗药物。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/191fd9a6990e/JCMM-24-11922-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/383d5528bfd8/JCMM-24-11922-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/efbce2427132/JCMM-24-11922-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/3f16becd5a4f/JCMM-24-11922-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/528eb3e18809/JCMM-24-11922-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/c762357a58bc/JCMM-24-11922-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/f1fd2cac21c5/JCMM-24-11922-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/191fd9a6990e/JCMM-24-11922-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/383d5528bfd8/JCMM-24-11922-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/efbce2427132/JCMM-24-11922-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/3f16becd5a4f/JCMM-24-11922-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/528eb3e18809/JCMM-24-11922-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/c762357a58bc/JCMM-24-11922-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/f1fd2cac21c5/JCMM-24-11922-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/41a8/7579697/191fd9a6990e/JCMM-24-11922-g007.jpg

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2
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3
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Nat Prod Bioprospect. 2021 Aug;11(4):365-377. doi: 10.1007/s13659-021-00298-w. Epub 2021 Feb 3.
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