Anses, Laboratory of Fougeres, European Union Reference Laboratory (EU-RL) for Antimicrobial and Dye Residue Control in Food-Producing Animals, Bâtiment Bioagropolis , Fougères, France.
Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2020 Oct;37(10):1651-1666. doi: 10.1080/19440049.2020.1778191. Epub 2020 Sep 1.
Colistin is a polypeptide antibiotic mainly used in porcine and poultry to treat gastrointestinal infections. It has been included by the World Health Organisation (WHO) in the list of critically important human antibiotics of high priority for antimicrobial resistance since 2017. Therefore, it is necessary to develop specific and sensitive screening methods for this molecule. Screening for colistin with immunoassays is an interesting alternative to LC-MS/MS screening methods. The performance of three commercially available ELISA kits was evaluated in poultry and porcine muscles for the detection of colistin in regards to its European maximum residue limit (MRL) (150 µg/kg). The applicability of the three ELISA kits to the detection of colistin at or below the MRL in porcine and poultry muscles was demonstrated. The detection capabilities (CCβ) of two kits were or lower than or equal to the MRL (150 µg/kg). The lowest detection capability (30 µg/kg) was achieved with the third ELISA kit. The specificity of the three kits was very satisfactory (false positive rates 0%). The three kits are very specific for the detection of colistin (colistin A and B) and polymyxin B.
黏菌素是一种多肽类抗生素，主要用于治疗猪和家禽的胃肠道感染。自 2017 年以来，世界卫生组织（WHO）已将其列入高度优先考虑抗微生物药物耐药性的重要人类抗生素清单。因此，有必要开发针对该分子的特异性和敏感的筛选方法。免疫测定法筛选黏菌素是一种替代 LC-MS/MS 筛选方法的有趣选择。评估了三种市售 ELISA 试剂盒在禽肉和猪肉肌肉中检测黏菌素的性能，以确定其欧洲最大残留限量（MRL）（150μg/kg）。证明了这三种 ELISA 试剂盒适用于检测猪肉和禽肉中低于或等于 MRL 的黏菌素。两种试剂盒的检测能力（CCβ）等于或低于 MRL（150μg/kg）。第三种 ELISA 试剂盒的最低检测能力（30μg/kg）。三种试剂盒的特异性都非常令人满意（假阳性率为 0%）。这三种试剂盒非常特异性地检测黏菌素（黏菌素 A 和 B）和多黏菌素 B。
