Ngan B Y, Chen-Levy Z, Weiss L M, Warnke R A, Cleary M L
Department of Pathology, Stanford University School of Medicine, CA 94305.
N Engl J Med. 1988 Jun 23;318(25):1638-44. doi: 10.1056/NEJM198806233182502.
For many non-Hodgkin's lymphomas, the bcl-2 gene has been implicated as a likely proto-oncogene, since it is consistently located at or near the breakpoint sites of t(14;18) chromosomal translocations. To define the role of the protein product of the bcl-2 gene in lymphoid cancers, we used anti-bcl-2 antibodies to perform immunohistochemical studies of frozen sections of 136 tissue specimens affected by lymphoma or non-neoplastic lymphoid disorders. Immunoreactive bcl-2 protein was observed in the neoplastic cells in almost all the follicular lymphomas, whereas no bcl-2 protein was detected in follicles affected by non-neoplastic processes or in normal lymphoid tissue. Every tumor with molecular-genetic evidence of t(14;18) translocation expressed detectable levels of bcl-2 protein, regardless of whether the breakpoint was located in or at a distance from the bcl-2 gene. These data show consistent expression of a proto-oncogenic protein in a large proportion of non-Hodgkin's lymphomas and provide further support of a role for bcl-2 in the pathogenesis of all lymphomas with the t(14;18) karyotypic abnormality. Increased expression of bcl-2 after t(14;18) translocations may be a specific marker for B-cell cancers, and demonstration of the protein with use of anti-bcl-2 antibodies could be useful in the diagnosis of many non-Hodgkin's lymphomas.
对于许多非霍奇金淋巴瘤，bcl-2基因被认为可能是一种原癌基因，因为它总是位于t(14;18)染色体易位的断点处或其附近。为了确定bcl-2基因的蛋白质产物在淋巴癌中的作用，我们使用抗bcl-2抗体对136份受淋巴瘤或非肿瘤性淋巴疾病影响的组织标本的冰冻切片进行免疫组织化学研究。在几乎所有滤泡性淋巴瘤的肿瘤细胞中都观察到了免疫反应性bcl-2蛋白，而在受非肿瘤性病变影响的滤泡或正常淋巴组织中未检测到bcl-2蛋白。每一个有t(14;18)易位分子遗传学证据的肿瘤都表达可检测水平的bcl-2蛋白，无论断点是位于bcl-2基因内还是与该基因有一定距离。这些数据表明，在很大一部分非霍奇金淋巴瘤中原癌基因蛋白表达一致，并为bcl-2在所有具有t(14;18)核型异常的淋巴瘤发病机制中的作用提供了进一步支持。t(14;18)易位后bcl-2表达增加可能是B细胞癌的一个特异性标志物，使用抗bcl-2抗体检测该蛋白可能有助于许多非霍奇金淋巴瘤的诊断。
