唾液和水平作为口腔鳞状细胞癌诊断的生物标志物。
Salivary and Levels as Biomarkers for Oral Squamous Cell Carcinoma Diagnosis.
机构信息
Department of Maxillofacial Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan.
Department of Surgery, School of Dentistry, Aichi-gakuin University Graduate School of Medicine, Nagoya, Japan.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2353-2361. doi: 10.21873/invivo.12048.
BACKGROUND/AIM: Oral cancer may become advanced because of delay in diagnosis. In order to promote oral cancer screening, simple and highly reliable screening methods that can be implemented at general dental clinics are required. Herein we investigated differential salivary gene expression between oral squamous cell carcinoma (OSCC) patients and healthy volunteers (HV) to identify new biomarkers for OSCC detection.
MATERIALS AND METHODS
Candidate genes were selected by microarrays, nuclear undecaprenyl pyrophosphate synthase 1 (NUS1) and reticulocalbin 1 (RCN1) were selected for further investigation. We used real-time quantitative reverse transcription PCR (qRT-PCR) to determine NUS1 and RCN1 expression levels in saliva and tissues.
RESULTS
qRT-PCR analysis of clinical samples revealed that OSCC patients had significantly higher expression of salivary NUS1 and RCN1 than HV.
CONCLUSION
A combination of NUS1 and RCN1 accurately distinguished patients from controls, and this combination can be implemented as a screening test for OSCC.
背景/目的：口腔癌可能因诊断延误而进展。为了促进口腔癌筛查，需要在普通牙科诊所实施简单且高度可靠的筛查方法。在此，我们研究了口腔鳞状细胞癌 (OSCC) 患者和健康志愿者 (HV) 之间唾液差异基因表达，以鉴定用于 OSCC 检测的新生物标志物。
材料和方法
通过微阵列选择候选基因，选择核十一碳烯基焦磷酸合酶 1 (NUS1) 和网质蛋白 1 (RCN1) 进行进一步研究。我们使用实时定量逆转录聚合酶链反应 (qRT-PCR) 确定唾液和组织中的 NUS1 和 RCN1 表达水平。
结果
临床样本的 qRT-PCR 分析显示，OSCC 患者的唾液 NUS1 和 RCN1 表达明显高于 HV。
结论
NUS1 和 RCN1 的组合能够准确地区分患者和对照者，该组合可作为 OSCC 的筛查试验。
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