抗坏血酸和 U-74389G 对大鼠肾缺血再灌注损伤的影响。
The Effects of Ascorbic Acid and U-74389G on Renal Ischemia-Reperfusion Injury in a Rat Model.
机构信息
Experimental, Educational and Research Center ELPEN, Athens, Greece.
1 Department of Propaedeutic Surgery, Hippokration Hospital, Athens, Greece.
出版信息
In Vivo. 2020 Sep-Oct;34(5):2475-2484. doi: 10.21873/invivo.12063.
BACKGROUND/AIM: U-74389G and ascorbic acid protect the cells from oxidation. This study aimed to depict their role in ischemia-reperfusion injury in a renal rat model.
MATERIALS AND METHODS
Sixty Wistars rats were randomized into six groups of 10 animals each. Group A Ischemia 30 min, reperfusion 60 min; Group B Ischemia 30 min, reperfusion 120 min; Group C Ischemia 30 min, ascorbic acid administration, reperfusion 60 min; Group D Ischemia 30 min, ascorbic acid administration, reperfusion 120 min; Group E Ischemia 30 min, U-74389G administration, reperfusion 60 min; Group F Ischemia 30 min, U-74389G administration, reperfusion 120 min. We then collected tissue and blood samples.
RESULTS
Histology and the significantly decreased malondialdehyde and tumor necrosis factor-α levels indicated that ascorbic acid was superior to U-74389G, at pre-defined time intervals.
CONCLUSION
Ascorbic acid and U-74389G ameliorated renal damage induced by ischemia-reperfusion injury, suggesting a therapeutic effect.
背景/目的：U-74389G 和抗坏血酸可保护细胞免受氧化损伤。本研究旨在描述它们在肾大鼠模型缺血再灌注损伤中的作用。
材料和方法
将 60 只 Wistar 大鼠随机分为 6 组，每组 10 只。A 组缺血 30 分钟，再灌注 60 分钟；B 组缺血 30 分钟，再灌注 120 分钟；C 组缺血 30 分钟，给予抗坏血酸，再灌注 60 分钟；D 组缺血 30 分钟，给予抗坏血酸，再灌注 120 分钟；E 组缺血 30 分钟，给予 U-74389G，再灌注 60 分钟；F 组缺血 30 分钟，给予 U-74389G，再灌注 120 分钟。然后收集组织和血液样本。
结果
组织学和丙二醛及肿瘤坏死因子-α水平的显著降低表明，在预先设定的时间间隔内，抗坏血酸优于 U-74389G。
结论
抗坏血酸和 U-74389G 改善了缺血再灌注损伤引起的肾损伤，提示具有治疗作用。
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