Park Eunkuk, Lee Chang Gun, Kim Jeonghyun, Lim Eunguk, Hwang Seokjin, Yun Seung Hee, Yong Yoonjoong, Jeong Hyesoo, Kim Ji Ae, Jin Hyun-Seok, Jeong Seon-Yong
Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, Korea.
Department of Biomedical Sciences, Ajou University Graduate School of Medicine, Suwon 16499, Korea.
Plants (Basel). 2020 Aug 28;9(9):1114. doi: 10.3390/plants9091114.
Osteoporosis is a porous bone disease caused by bone density loss, which increases the risk of fractures. (CO) and (AJ) have been used as traditional herbal medicine for various disorders in East Asia. Although the anti-osteoporotic effects of single extract of CO and AJ have already been reported, the synergistic effect of a combined mixture has not been studied. In this study, we investigated the effects of a CO and AJ herbal mixture on osteoporosis in in vitro and in vivo models. The results demonstrate that treatment with the CO and AJ mixture significantly promoted osteoblast differentiation of MC3T3-E1 mouse preosteoblasts through the upregulation of osteoblastic differentiation-associated genes such as alkaline phosphatase (), runt-related transcription factor 2 (), and bone gamma-carboxyglutamic acid-containing protein () while the mixture significantly inhibited differentiation of osteoclasts isolated from primary-cultured mouse monocytes. In addition, oral administration of CO and AJ mixture significantly prevented bone mineral density loss and trabecular bone structures in an ovariectomy-induced osteoporotic mouse model. These results suggest that the combination treatment of CO and AJ mixture might be a beneficial therapy for osteoporosis.
骨质疏松症是一种因骨密度降低引起的骨多孔性疾病，会增加骨折风险。在东亚地区，（此处原文中“(CO) and (AJ)”表述不完整，推测可能是两种草药名称的缩写，假设为“黄连（CO）和艾叶（AJ）”）黄连（CO）和艾叶（AJ）已被用作治疗各种疾病的传统草药。尽管已有报道称黄连（CO）和艾叶（AJ）的单一提取物具有抗骨质疏松作用，但它们混合后的协同作用尚未得到研究。在本研究中，我们在体外和体内模型中研究了黄连（CO）和艾叶（AJ）草药混合物对骨质疏松症的影响。结果表明，黄连（CO）和艾叶（AJ）混合物处理通过上调成骨细胞分化相关基因，如碱性磷酸酶（）、 runt相关转录因子2（）和骨γ-羧基谷氨酸蛋白（），显著促进了MC3T3-E1小鼠前成骨细胞的成骨细胞分化，同时该混合物显著抑制了从原代培养的小鼠单核细胞中分离出的破骨细胞的分化。此外，在去卵巢诱导的骨质疏松小鼠模型中，口服黄连（CO）和艾叶（AJ）混合物可显著预防骨矿物质密度损失和小梁骨结构破坏。这些结果表明，黄连（CO）和艾叶（AJ）混合物联合治疗可能是一种治疗骨质疏松症的有益疗法。
