Cold Spring Harb Protoc. 2020 Sep 1;2020(9):101683. doi: 10.1101/pdb.prot101683.
This protocol is for isolating total RNA from either larvae (L1-L4) or adult worms using a monophasic lysis reagent. It is preferable when multiple samples have to be processed.
本方案通过单相裂解试剂从幼虫(L1-L4)或成虫中提取总 RNA。当需要处理多个样本时,该方法较为适用。