Jiang Shulun, Yang Jian, Fang Dian
Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, People's Republic of China.
Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, People's Republic of China.
R Soc Open Sci. 2020 Jul 15;7(7):191356. doi: 10.1098/rsos.191356. eCollection 2020 Jul.
Derived from polycyclic aromatic hydrocarbons (PAHs), oxygenated-PAHs (oxy-PAHs) may pose hazards to aquatic organisms, which remain largely unknown. is an important anadromous fish species of high economic and ecological values. In the present study, was acutely exposed to 44.29 µg l 9,10-phenanthrenequione (9,10-PQ) for 96 h. Changes of antioxidant indices and metabolite profiles in plasma were compared between 9,10-PQ treatment and the control. The results showed that 9,10-PQ treatment significantly increased malondialdehyde (MDA) content during 6 to 96 h, increased superoxide dismutase (SOD) and catalase (CAT) activities at 6 h, but decreased them at 96 h. These results indicated that 9,10-PQ induced oxidative stress to fish. Ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) analysis revealed that four metabolic pathways were influenced in response to treatment with 9,10-PQ, including glycerophospholipid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, purine metabolism and sulfur metabolism. These pathways are associated with antioxidant mechanisms, biosynthesis of neurotransmitters and innate immune functions. Thus, the as-obtained results confirmed that 9,10-PQ induced oxidative stress and raised concerns of neurotoxicity and immunotoxicity to fish. Overall, the present study posed a high environmental risk of oxy-PAHs to aquatic ecosystems.
含氧多环芳烃（oxy-PAHs）源自多环芳烃（PAHs），可能对水生生物构成危害，而这在很大程度上仍不为人知。[某种鱼]是一种具有重要经济和生态价值的溯河洄游鱼类。在本研究中，[某种鱼]被急性暴露于44.29微克/升的9,10-菲醌（9,10-PQ）中96小时。比较了9,10-PQ处理组和对照组血浆中抗氧化指标和代谢物谱的变化。结果表明，9,10-PQ处理在6至96小时内显著增加了丙二醛（MDA）含量，在6小时时增加了超氧化物歧化酶（SOD）和过氧化氢酶（CAT）的活性，但在96小时时降低了它们的活性。这些结果表明9,10-PQ对鱼类诱导了氧化应激。超高效液相色谱-质谱联用（UPLC-MS）分析显示，有四条代谢途径受到9,10-PQ处理的影响，包括甘油磷脂代谢、苯丙氨酸、酪氨酸和色氨酸生物合成、嘌呤代谢和硫代谢。这些途径与抗氧化机制、神经递质生物合成和先天免疫功能有关。因此，所获得的结果证实9,10-PQ诱导了氧化应激，并引发了对鱼类神经毒性和免疫毒性的担忧。总体而言，本研究表明oxy-PAHs对水生生态系统具有很高的环境风险。
