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使用碱性成纤维细胞生长因子-肾病结构域-胶原结合结构域融合蛋白联合同种异体脱矿骨粉加速骨折愈合过程中骨痂的形成。

Acceleration of callus formation during fracture healing using basic fibroblast growth factor-kidney disease domain-collagen-binding domain fusion protein combined with allogenic demineralized bone powder.

作者信息

Saito Wataru, Uchida Kentaro, Matsushita Osamu, Inoue Gen, Sekiguchi Hiroyuki, Aikawa Jun, Fujimaki Hisako, Takaso Masashi

机构信息

Department of Orthopaedic Surgery, Kitasato University School of Medicine, 1-15-1 Minami-ku Kitasato, Sagamihara, Kanagawa, Japan.

Department of Bacteriology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Kita-ku Shikata-cho, Okayama, Japan.

出版信息

J Orthop Surg Res. 2015 May 9;10:59. doi: 10.1186/s13018-015-0201-0.

DOI:10.1186/s13018-015-0201-0
PMID:25956801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC4429668/
Abstract

BACKGROUND

To repair fractures with large bone defects or gaps, demineralized allogenic bone matrix (DBM) is often applied to the fracture site. However, studies have shown that the use of DBM alone has limited efficacy for repairing fractures. In the present study, we developed an allogenic demineralized bone powder (DBP) with basic fibroblast-derived growth factor containing a polycystic kidney disease (PKD) domain and collagen-binding domain (CBD) from Clostridium histolyticum collagenase (ColH) and investigated the stimulatory effects of bFGF-PKD-CBD combined with allogenic DBP on bone growth in a mouse femur fracture model.

METHODS

DBP mixed with either phosphate-buffered saline (PBS) (DBP/PBS), 0.58 nmol basic fibroblast growth factor (bFGF) (0.58 nmol DBP/bFGF), 0.058 nmol bFGF-PKD-CBD (0.058 nmol DBP/bFGF-PKD-CBD), or 0.58 nmol bFGF-PKD-CBD (0.58 nmol DBP/bFGF-PKD-CBD) was grafted into fracture sites.

RESULTS

bFGF-PKD-CBD/DBP composite accelerates callus formation in a bone fracture model in mice and clearly showed that the composite also increases bone mineral density at fracture sites compared to bFGF/DBP. In addition, bFGF-PKD-CBD/DBP increased callus volume and bone mineral content to similar levels in fractures treated with a tenfold higher amount of bFGF at 4 weeks.

CONCLUSIONS

Our results suggest that bFGF-PKD-CBD/DBP may be useful for promoting fracture healing in the clinical setting.

摘要

背景

为修复大的骨缺损或间隙骨折，常将脱矿异体骨基质（DBM）应用于骨折部位。然而，研究表明单独使用DBM修复骨折的疗效有限。在本研究中，我们开发了一种异体脱矿骨粉（DBP），其含有来自溶组织梭菌胶原酶（ColH）的含多囊肾病（PKD）结构域和胶原结合结构域（CBD）的碱性成纤维细胞衍生生长因子，并在小鼠股骨骨折模型中研究了bFGF-PKD-CBD与异体DBP联合对骨生长的刺激作用。

方法

将DBP与磷酸盐缓冲盐水（PBS）（DBP/PBS）、0.58 nmol碱性成纤维细胞生长因子（bFGF）（0.58 nmol DBP/bFGF）、0.058 nmol bFGF-PKD-CBD（0.058 nmol DBP/bFGF-PKD-CBD）或0.58 nmol bFGF-PKD-CBD（0.58 nmol DBP/bFGF-PKD-CBD）混合后植入骨折部位。

结果

bFGF-PKD-CBD/DBP复合物在小鼠骨折模型中加速了骨痂形成，并且清楚地表明，与bFGF/DBP相比，该复合物还增加了骨折部位的骨矿物质密度。此外，在4周时，bFGF-PKD-CBD/DBP使骨痂体积和骨矿物质含量增加到用十倍量更高的bFGF治疗的骨折中的相似水平。

结论

我们的结果表明，bFGF-PKD-CBD/DBP可能有助于在临床环境中促进骨折愈合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ffc/4429668/5b72bed8a383/13018_2015_201_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ffc/4429668/ec0ce31396f3/13018_2015_201_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ffc/4429668/5b72bed8a383/13018_2015_201_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ffc/4429668/ec0ce31396f3/13018_2015_201_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/3ffc/4429668/5b72bed8a383/13018_2015_201_Fig2_HTML.jpg

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