Teka Brhanu, Gizaw Muluken, Ruddies Friederike, Addissie Adamu, Chanyalew Zewditu, Skof Anna Sophie, Thies Sarah, Mihret Adane, Kantelhardt Eva Johanna, Kaufmann Andreas M, Abebe Tamrat
Department of Microbiology, Immunology and Parasitology School of Medicine College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
Addis Ababa University, School of Public Health, Department of Preventive Medicine, Addis Ababa, Ethiopia.
Int J Cancer. 2021 Feb 1;148(3):723-730. doi: 10.1002/ijc.33278. Epub 2020 Sep 8.
In Ethiopia, cervical cancer is the second leading cause of morbidity and mortality from all cancers in women. Persistent infection with human papillomaviruses (HPV) plays a key role in the development of cervical intraepithelial neoplasia and invasive cervical cancer. To establish baseline data on the population-based prevalence of HPV infection and genotype distribution, we investigated cervical HPV epidemiology among rural women. This population-based study was conducted among rural women aged 30-49 years in Butajira, south-central Ethiopia. A total of 893 samples were tested from 1020 screened women. A self-sampling device (Evalyn Brush, Rovers, Oss, The Netherlands) was used and HPV presence and genotype was determined using multiplexed genotyping (MPG) by BSGP5+/6+ PCR with Luminex read out. The HPV positivity rate was 23.2% (95% CI: 23.54-22.86%) and 20.5% (95% CI = 20.79-20.21) and 10.3% (95% CI = 10.52-10.08) women were high-risk (hr- and low-risk (lr-) HPV positive, respectively. Fifty five (7.2%) of the women showed multiple hr-HPV infections. Age-specific hr-HPV infection peaked in the age-group 30- to 34 years old (58.6%) and decreased in 35-39, 40-44 and 45-49 years to 20.4%, 4.5% and 3.8% respectively. The top five prevalent hr-HPV genotypes were HPV16 (57.1%), 35 (20.3%), 52 (15.8%), 31 (14.1%), and 45 (9.6%) in the Butajira district. As a first population-based study in the country, our results can serve as valuable reference to guide nationwide cervical cancer screening and HPV vaccination programs in Ethiopia.
在埃塞俄比亚，宫颈癌是女性所有癌症中导致发病和死亡的第二大主要原因。人乳头瘤病毒（HPV）持续感染在宫颈上皮内瘤变和浸润性宫颈癌的发展中起关键作用。为建立基于人群的HPV感染患病率和基因型分布的基线数据，我们对农村女性的宫颈HPV流行病学进行了调查。这项基于人群的研究在埃塞俄比亚中南部布塔吉拉30至49岁的农村女性中开展。共对1020名接受筛查的女性的893份样本进行了检测。使用了一种自采样装置（Evalyn Brush，荷兰罗孚公司，奥斯市），并通过带有Luminex读数的BSGP5+/6+ PCR多重基因分型（MPG）来确定HPV的存在及基因型。HPV阳性率为23.2%（95%置信区间：23.54 - 22.86%），高危（hr-）和低危（lr-）HPV阳性的女性分别占20.5%（95%置信区间 = 20.79 - 20.21）和10.3%（95%置信区间 = 10.52 - 10.08）。55名（7.2%）女性呈现多重hr-HPV感染。特定年龄组的hr-HPV感染在30至34岁年龄组达到峰值（58.6%），在35 - 39岁、40 - 44岁和45 - 49岁分别降至20.4%、4.5%和3.8%。在布塔吉拉区，最常见的五种hr-HPV基因型为HPV16（57.1%）、35（20.3%）、52（15.8%）、31（14.1%）和45（9.6%）。作为该国第一项基于人群的研究，我们的结果可为指导埃塞俄比亚全国宫颈癌筛查和HPV疫苗接种计划提供有价值的参考。
