Teka Brhanu, Yoshida-Court Kyoko, Firdawoke Ededia, Chanyalew Zewditu, Gizaw Muluken, Addissie Adamu, Mihret Adane, Colbert Lauren E, Napravnik Tatiana Cisneros, El Alam Molly B, Lynn Erica J, Mezzari Melissa, Anuja Jhingran, Kantelhardt Eva Johanna, Kaufmann Andreas M, Klopp Ann H, Abebe Tamrat
Department of Microbiology, Immunology and Parasitology School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa P.O. Box 9086, Ethiopia.
Global Health Working Group, Martin-Luther-University, Halle-Wittenberg, 06097 Halle, Germany.
Microorganisms. 2023 Mar 24;11(4):833. doi: 10.3390/microorganisms11040833.
Although high-risk human papillomavirus infection is a well-established risk factor for cervical cancer, other co-factors within the local microenvironment may play an important role in the development of cervical cancer. The current study aimed to characterize the cervicovaginal microbiota in women with premalignant dysplasia or invasive cervical cancer compared with that of healthy women. The study comprised 120 Ethiopian women (60 cervical cancer patients who had not received any treatment, 25 patients with premalignant dysplasia, and 35 healthy women). Cervicovaginal specimens were collected using either an Isohelix DNA buccal swab or an Evalyn brush, and ribosomal RNA sequencing was used to characterize the cervicovaginal microbiota. Shannon and Simpson diversity indices were used to evaluate alpha diversity. Beta diversity was examined using principal coordinate analysis of weighted UniFrac distances. Alpha diversity was significantly higher in patients with cervical cancer than in patients with dysplasia and in healthy women ( < 0.01). Beta diversity was also significantly different in cervical cancer patients compared with the other groups (weighted UniFrac Bray-Curtis, < 0.01). Microbiota composition differed between the dysplasia and cervical cancer groups. was particularly enriched in patients with cancer, and a high relative abundance of species was identified in the dysplasia and healthy groups, whereas , , , and species predominated in the cervical cancer group. In summary, we identified differences in cervicovaginal microbiota diversity, composition, and relative abundance between women with cervical cancer, women with dysplasia, and healthy women. Additional studies need to be carried out in Ethiopia and other regions to control for variation in sample collection.
虽然高危型人乳头瘤病毒感染是宫颈癌公认的危险因素，但局部微环境中的其他协同因素可能在宫颈癌的发生发展中起重要作用。本研究旨在比较癌前发育异常或浸润性宫颈癌女性与健康女性的宫颈阴道微生物群特征。该研究纳入了120名埃塞俄比亚女性（60名未接受任何治疗的宫颈癌患者、25名癌前发育异常患者和35名健康女性）。使用Isohelix DNA口腔拭子或Evalyn刷收集宫颈阴道标本，并采用核糖体RNA测序来表征宫颈阴道微生物群。使用香农和辛普森多样性指数评估α多样性。使用加权UniFrac距离的主坐标分析来检查β多样性。宫颈癌患者的α多样性显著高于发育异常患者和健康女性（<0.01）。与其他组相比，宫颈癌患者的β多样性也有显著差异（加权UniFrac Bray-Curtis，<0.01）。发育异常组和宫颈癌组的微生物群组成不同。在癌症患者中特别富集，在发育异常组和健康组中鉴定出高相对丰度的物种，而在宫颈癌组中，物种、、和占主导地位。总之，我们确定了宫颈癌女性、发育异常女性和健康女性之间宫颈阴道微生物群多样性、组成和相对丰度的差异。需要在埃塞俄比亚和其他地区开展更多研究，以控制样本采集的差异。
