Department of Oral Health Practice, College of Dentistry, Center for Oral Health Research, University of Kentucky, Lexington, KY, USA.
Division of Orthodontics, Department of Oral Health Science, College of Dentistry, University of Kentucky, Lexington, KY, USA.
J Clin Periodontol. 2020 Nov;47(11):1317-1325. doi: 10.1111/jcpe.13361. Epub 2020 Oct 8.
Previous data from our laboratory have demonstrated that localized aggressive periodontitis (LAP) patients produce elevated levels of pro-inflammatory cytokines in response to TLR4 and TLR2 ligation compared to unrelated and periodontally healthy controls (HC). The aim of the present work is to evaluate the contribution of TLR-related gene expression and miRNA regulation in LAP disease.
Peripheral blood mononuclear cells (PBMCs) from LAP and health control (HC) patients were isolated. Gene and miRNA expression involved in TLR signalling pathway and immunopathology were evaluated in unstimulated PBMCs by real-time PCR (RT-PCR).
TICAM-1 (TRIF), FOS, IRAK1, TLR2 and CCL2 genes and the miRNAs miR-9-5p, miR-155-5p and 203a-3p, miR-147a, miR-182-5p and miR-183-5p were significantly up-regulated in LAP compared to HC.
Most of the genes and miRNAs overexpressed here are directly or indirectly related to immune response and inflammation. This profile supports our previous findings that suggests LAP patients have a "hyper-responsive" phenotype upon activation of TLR pathway by periodontal pathogens.
本实验室先前的数据表明，与无关个体和牙周健康对照（HC）相比，局部侵袭性牙周炎（LAP）患者在 TLR4 和 TLR2 配体作用下产生更高水平的促炎细胞因子。本研究旨在评估 TLR 相关基因表达和 miRNA 调节在 LAP 疾病中的作用。
分离 LAP 和健康对照（HC）患者的外周血单核细胞（PBMC）。通过实时 PCR（RT-PCR）评估未刺激的 PBMC 中 TLR 信号通路和免疫病理学相关的基因和 miRNA 表达。
TICAM-1（TRIF）、FOS、IRAK1、TLR2 和 CCL2 基因以及 miR-9-5p、miR-155-5p 和 203a-3p、miR-147a、miR-182-5p 和 miR-183-5p 等 miRNA 在 LAP 中显著上调。
此处过度表达的大多数基因和 miRNA 直接或间接地与免疫反应和炎症有关。该特征支持我们之前的发现，即提示 LAP 患者在牙周病原体激活 TLR 途径时具有“高反应性”表型。
