Nandipati Sowmya Reddy, Appukuttan Devapriya, Subramanian Sangeetha, Prakash P S G
Department of Periodontics, SRM Dental College and Hospital, Barathi Salai, Ramapuram, Chennai, 600089, India.
J Genet Eng Biotechnol. 2022 Dec 2;20(1):161. doi: 10.1186/s43141-022-00441-1.
Monocyte-macrophage lineage cells are committed towards osteoclast differentiation in vitro by the downregulation of microphthalmia-induced transcription factor (MITF) by miRNA-155. Therefore, we aimed to evaluate miRNA-155 expression and explore the regulation of MITF by miRNA-155 during osteoclastogenesis in periodontitis.
Ninety-eight subjects were recruited and categorized into the following: group I (cases)-systemically healthy with localized stage III/IV periodontitis (N = 49) and group II (controls)-systemically and periodontally healthy (N = 49). Gingival tissue samples were procured and qRT-PCR analysis was carried out for relative gene expression.
The mean ΔCT of miRNA-155 expression was -1.04 ± 2.26 and -0.01 ± 1.4 respectively for groups I and II. There was a statistically significant difference in the miRNA-155 expression (P ≤ 0.01) between the groups. The mean ΔCT of MITF expression for groups I and II was 4.15± 2.16 and 3.51± 1.57 respectively with no significant difference (P > 0.01) between the groups. In the periodontitis group, miRNA-155 expression increased by fivefolds (P ≤ 0.01) whereas MITF expression showed no significant difference in the fold change between the groups (P > 0.01). The site-specific clinical parameters showed a statistically significant strong negative and positive correlation with the ΔCT and fold change values of miRNA-155 respectively in the total 98 samples (P < 0.01). miRNA-155 was able to discriminate between periodontal health and disease with a diagnostic accuracy of 96.9% (95%CI: 91.38-98.95) and the AUC was 0.98 (95%CI: 0.97-1.0, SE = 0.008, P < 0.001) in ROC analysis with a sensitivity of 93.8% (95%CI: 83.48-97.9) and specificity of 100% (95%CI: 92.73-100).
miRNA-155 was dysregulated and upregulated by fivefolds in periodontal disease. It can be used as a potential biomarker to discriminate between periodontal health and disease. No difference in the MITF gene expression was demonstrated between periodontal health and disease. The result suggested that miRNA-155 does not affect the expression of MITF gene in the process of osteoclastogenesis in localized stage III/IV periodontitis within this study design and limitations.
单核细胞 - 巨噬细胞系细胞在体外通过微小RNA - 155（miRNA - 155）下调小眼畸形相关转录因子（MITF）而向破骨细胞分化。因此，我们旨在评估miRNA - 155的表达，并探讨在牙周炎破骨细胞形成过程中miRNA - 155对MITF的调控作用。
招募了98名受试者，并分为以下两组：第一组（病例组）——全身健康但患有局限性III/IV期牙周炎（N = 49）；第二组（对照组）——全身及牙周均健康（N = 49）。采集牙龈组织样本，并进行qRT - PCR分析以检测相对基因表达。
第一组和第二组miRNA - 155表达的平均ΔCT分别为 - 1.04±2.26和 - 0.01±1.4。两组间miRNA - 155表达存在统计学显著差异（P≤0.01）。第一组和第二组MITF表达的平均ΔCT分别为4.15±2.16和3.51±1.57，两组间无显著差异（P>0.01）。在牙周炎组中，miRNA - 155表达增加了五倍（P≤0.01），而两组间MITF表达的倍数变化无显著差异（P>0.01）。在总共98个样本中，位点特异性临床参数分别与miRNA - 155的ΔCT和倍数变化值呈现统计学显著的强负相关和正相关（P<0.01）。miRNA - 155能够区分牙周健康和疾病，诊断准确率为96.9%（95%CI：91.38 - 98.95），在ROC分析中AUC为0.98（95%CI：0.97 - 1.0，SE = 0.008，P<0.001），敏感性为93.8%（95%CI：83.48 - 97.9），特异性为100%（95%CI：92.73 - 100）。
在牙周疾病中，miRNA - 155失调且上调了五倍。它可作为区分牙周健康和疾病的潜在生物标志物。牙周健康和疾病之间未显示出MITF基因表达的差异。结果表明，在本研究设计和局限性范围内，miRNA - 155在局限性III/IV期牙周炎破骨细胞形成过程中不影响MITF基因的表达。
