Department of Oncology, Wuxi People's Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu, China.
DNA Cell Biol. 2020 Oct;39(10):1838-1849. doi: 10.1089/dna.2020.5644. Epub 2020 Sep 1.
The breast cancer gene 1 (BRCA1) is a tumor suppressor, and mutations or epigenetic inactivation will increase the risk of breast cancer oncogenesis. The current research aimed to explore the relationship between BRCA1 expression, prognosis, and tumor immunity in hepatocellular carcinoma (HCC). In this study, BRCA1 expression was analyzed via multiple online databases and its association with clinical characteristics, prognosis and genetic alterations was identified using the original The Cancer Genome Atlas-liver hepatocellular carcinoma cohorts. DNA methylation sites and their prognostic values were analyzed using MethSurv. The correlations between BRCA1 and immune infiltration were investigated via Tumor Immune Estimation Resource. As results, BRCA1 was significantly upregulated in tumor tissues in multiple HCC cohorts. Besides, high BRCA1 expression was correlated with race, advanced T stage, clinical stage, poor tumor grade, MSI status, and worse prognosis. Notably, BRCA1 expression was positively correlated with infiltration levels of B cells, CD4+ T cells, CD8+ T cells, neutrophils, macrophages, and dendritic cells. The current findings imply that BRCA1 is associated with prognosis and immune infiltration, laying foundations for in-depth research on the role of BRCA1 in HCC.
乳腺癌基因 1（BRCA1）是一种肿瘤抑制基因，其突变或表观遗传失活会增加乳腺癌发生肿瘤的风险。本研究旨在探讨 BRCA1 表达、预后与肝癌肿瘤免疫之间的关系。本研究通过多个在线数据库分析 BRCA1 表达，并利用原始癌症基因组图谱-肝癌队列鉴定其与临床特征、预后和遗传改变的关系。采用 MethSurv 分析 DNA 甲基化位点及其预后价值。通过肿瘤免疫评估资源探讨 BRCA1 与免疫浸润的相关性。结果显示，BRCA1 在多个 HCC 队列的肿瘤组织中显著上调。此外，高 BRCA1 表达与种族、晚期 T 分期、临床分期、较差的肿瘤分级、微卫星不稳定性状态和预后不良相关。值得注意的是，BRCA1 表达与 B 细胞、CD4+T 细胞、CD8+T 细胞、中性粒细胞、巨噬细胞和树突状细胞的浸润水平呈正相关。本研究结果提示 BRCA1 与预后和免疫浸润相关，为深入研究 BRCA1 在 HCC 中的作用奠定了基础。
