Vinge E, Ekman R
Department of Clinical Pharmacology, University Hospital, Lund, Sweden.
Ther Drug Monit. 1988;10(1):8-15.
Urinary samples were collected from individuals not taking cardiac glycosides. Aliquots of 30 ml were passed through preparative octadecylsilane-bonded phase columns and eluted in fractions by stepwise increasing concentrations of acetonitrile. Eluted fractions were analysed for their contents of endogenous digoxinlike substance (EDLS) by radioimmunoassay of digoxin and by a bioassay of cardiac glycosides, which measures the uptake of rubidium (86Rb) by erythrocytes as an index of Na+, K+-ATPase activity. In both assays, digoxinlike activity was found in several fractions, but the highest values were consistently measured in the fractions eluted with 40% acetonitrile. Greater amounts of EDLS were recovered from the urine of pregnant women than from the urine of men and nonpregnant women.
从未服用强心苷的个体中收集尿液样本。取30毫升等分试样通过制备型十八烷基硅烷键合相柱，并通过逐步增加乙腈浓度进行分步洗脱。通过地高辛放射免疫测定法和强心苷生物测定法分析洗脱组分中的内源性地高辛样物质（EDLS）含量，该生物测定法以红细胞对铷（86Rb）的摄取作为钠钾ATP酶活性指标来测量强心苷。在两种测定中，均在几个组分中发现了地高辛样活性，但在用40%乙腈洗脱的组分中始终测得最高值。从孕妇尿液中回收的EDLS量比从男性和未怀孕女性尿液中回收的量更多。
